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Pervasive, coordinated protein level changes driven by transcript isoform switching during meiosis in yeast

ABSTRACT: To better understand the gene regulatory mechanisms that program developmental processes, we carried out simultaneous, genome-wide measurements of mRNA, translation and protein through meiotic differentiation in budding yeast.

OTHER RELATED OMICS DATASETS IN: PRJNA428526

INSTRUMENT(S): Q Exactive

ORGANISM(S): Saccharomyces Cerevisiae (ncbitaxon:4932)

SUBMITTER: Marko Jovanovic

PROVIDER: MSV000081874 | MassIVE | Tue Jan 02 17:13:00 GMT 2018

REPOSITORIES: MassIVE

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Publications

Precise Post-translational Tuning Occurs for Most Protein Complex Components during Meiosis.

Eisenberg Amy Rose AR Higdon Andrea A Keskin Abdurrahman A Hodapp Stefanie S Jovanovic Marko M Brar Gloria Ann GA

Cell reports 20181201 13

Protein degradation is known to be a key component of expression regulation for individual genes, but its global impact on gene expression has been difficult to determine. We analyzed a parallel gene expression dataset of yeast meiotic differentiation, identifying instances of coordinated protein-level decreases to identify new cases of regulated meiotic protein degradation, including of ribosomes and targets of the meiosis-specific anaphase-promoting complex adaptor Ama1. Comparison of protein ...[more]

PMID: 30590036

Publication: 1/2

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Pervasive, coordinated protein level changes driven by transcript isoform switching during meiosis

Project description:To better understand the gene regulatory mechanisms that program developmental processes, we carried out simultaneous, genome-wide measurements of mRNA, translation and protein through meiotic differentiation in budding yeast. Surprisingly, we observed that the levels of several hundred mRNAs are anti-correlated with their corresponding protein products. We show that rather than arising from canonical forms of gene regulatory control, the regulation of at least 380 such cases—or over 8% of all measured genes—involves temporally regulated switching between production of a canonical, translatable transcript and a 5' extended isoform that is not efficiently translated into protein. By this pervasive mechanism for the modulation of protein levels through a natural developmental program, a single transcription factor can coordinately activate and repress protein synthesis for distinct sets of genes. The distinction is not based on whether an mRNA is induced or not, but rather based on the type of transcript produced.

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