Dataset Information

Youn_et_al_RNAbodies_SAINT3108_MSPLIT_DDA (PAIRED with MSV000081419)

ABSTRACT: This set of submissions contains the mass spectrometry files acquired using Data-Dependent Acquisition mode (DDA), which were paired with Data-Independent Acquisition mode (DIA) files (MSV000081419) for the manuscript by Ji-Young Youn et al. that describes the high-density proximity mapping of RNA bodies. These DDA files were used to generate spectral library in MSPLIT analysis of both DDA and DIA files. The paired SAINT 3108 set entails BioID experiments of stress granule and Processing-body baits expressed in Flp-In T-REx HEK293 cells, treated or untreated with sodium arsenite during labeling period.

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Anne-Claude Gingras

PROVIDER: MSV000081948 | MassIVE | Wed Jan 17 13:31:00 GMT 2018

REPOSITORIES: MassIVE

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Publications

High-Density Proximity Mapping Reveals the Subcellular Organization of mRNA-Associated Granules and Bodies.

Youn Ji-Young JY Dunham Wade H WH Hong Seo Jung SJ Knight James D R JDR Bashkurov Mikhail M Chen Ginny I GI Bagci Halil H Rathod Bhavisha B MacLeod Graham G Eng Simon W M SWM Angers Stéphane S Morris Quaid Q Fabian Marc M Côté Jean-François JF Gingras Anne-Claude AC

Molecular cell 20180125 3

mRNA processing, transport, translation, and ultimately degradation involve a series of dedicated protein complexes that often assemble into large membraneless structures such as stress granules (SGs) and processing bodies (PBs). Here, systematic in vivo proximity-dependent biotinylation (BioID) analysis of 119 human proteins associated with different aspects of mRNA biology uncovers 7424 unique proximity interactions with 1,792 proteins. Classical bait-prey analysis reveals connections of hundr ...[more]

PMID: 29395067

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