Proteomics

Dataset Information

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Hewings et al. 2018

ABSTRACT: raw data files associated with manuscript titled "Reactive-site-centric chemoproteomics identifies a distinct class of deubiquitinase enzymes". Result file includes all PSMs identified in the experiment after performing necessary quality filter.

INSTRUMENT(S): Orbitrap Fusion, LTQ Orbitrap Elite

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Ingrid Wertz  

PROVIDER: MSV000081972 | MassIVE | Thu Jan 25 09:23:00 GMT 2018

REPOSITORIES: MassIVE

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Publications

Activity-based probes (ABPs) are widely used to monitor the activity of enzyme families in biological systems. Inferring enzyme activity from probe reactivity requires that the probe reacts with the enzyme at its active site; however, probe-labeling sites are rarely verified. Here we present an enhanced chemoproteomic approach to evaluate the activity and probe reactivity of deubiquitinase enzymes, using bioorthogonally tagged ABPs and a sequential on-bead digestion protocol to enhance the ident  ...[more]

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