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(3S)-Lysyl hydroxylation of TRAFAC GTPases is catalyzed by the 1 Jumonji-C oxygenase JMJD7

ABSTRACT: Biochemical, structural, and cellular studies reveal Jumonji-C (JmjC) domain-containing (JMJD7) as a 2-oxoglutarate (2OG)-dependent oxygenase catalyzing a previously unreported type of post translational modification, (3S)-lysyl hydroxylation. Crystallographic analyses reveal JMJD7 as more closely related to the JmjC hydroxylases rather than the JmjC demethylases. Biophysical and mutation studies show that JMJD7 has a unique dimerization mode, with interactions between monomers involving both N- and C-terminal regions and disulfide bond formation. A proteomic approach identifies two related members of the Translation Factor (TRAFAC) family of GTPases, Developmentally Regulated GTP Binding Proteins 1 and 2 (DRG1/2), as activity-dependent JMJD7 interactors. Mass spectrometric analyses demonstrate that JMJD7 catalyzes Fe(II)- and 2OG-dependent hydroxylation of a highly-conserved lysine residue in DRG1/2; amino acid analyses reveal JMJD7 catalyzes (3S)-lysyl hydroxylation. The functional assignment of JMJD7 will enable future studies to define the role of DRG hydroxylation in cell growth and disease. MSQ506 - hydroxylation of DRG1/2 detected after assay with Jmjd7 wt, H178A mutant and empty vector (analysed with Q-Exactive classic, Progenesis QI and Mascot) MSQ784 - as above (analysed Q-Exactive classic, Progenesis QI and PEAKS) MSQ849 - Hydroxylation of endogenous DRG (analysed with Q-Exactive HF and PEAKS) MSQ884 - as above (analysed with Orbitrap Fusion Lumos and PEAKS)

INSTRUMENT(S): Orbitrap Fusion ETD

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Mathew Coleman

PROVIDER: MSV000082185 | MassIVE | Tue Mar 20 03:43:00 GMT 2018

SECONDARY ACCESSION(S): PXD009263

REPOSITORIES: MassIVE

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(3S)-Lysyl hydroxylation of TRAFAC GTPases is catalyzed by the 1 Jumonji-C oxygenase JMJD7

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