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Butyrate Suppresses Proliferation of Colon Cancer Cells via Targeting Pyruvate Kinase M2 and Metabolic Reprogramming

ABSTRACT: How butyrate interrupts the metabolism of cancer cells and leads to the suppression of cell proliferation remains unclear. We employed a metabolomics-proteomics combined approach to explore the link between butyrate-mediated proliferation arrest and cell metabolism. Metabolomics study revealed a remodeled metabolic profile with pronounced accumulation of pyruvate. A proteomics-based approach reveals PKM2 as a direct target of butyrate. it activates PKM2 via promoting its dephosphorylation and tetramerization.Our study provides a mechanistic link between PKM2-induced metabolic remodeling and the anti-tumorigenic function of butyrate.

INSTRUMENT(S): MALDI Synapt G2-S HDMS

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Hui Ye

PROVIDER: MSV000082189 | MassIVE | Wed Mar 21 02:33:00 GMT 2018

REPOSITORIES: MassIVE

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GNPS - Butyrate Suppresses Proliferation of Colon Cancer Cells via Targeting Pyruvate Kinase M2 and Metabolic Reprogramming

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