Chemical-mediated digestion: an alternative realm for middle-down proteomics?

ABSTRACT: Generating longer peptides is a paramount step in launching a middle-down proteomics pipeline, but the quest for the selection of a cleaving agent that would provide the desired 3-15 kDa peptides remains open. Here we describe the use of chemical reagent for a middle-down pipeline. Particularly we investigated CNBr, o-iodosobenzoic acid, BNPS-Skatole and, most importantly, NTCB as potential cleaving agents targeting rare amino acid residues.

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Bos Taurus (ncbitaxon:9913) Homo Sapiens (ncbitaxon:9606) Saccharomyces Cerevisiae (ncbitaxon:4932)

SUBMITTER: Yury O. Tsybin  

PROVIDER: MSV000082216 | MassIVE | Tue Mar 27 14:41:00 BST 2018

REPOSITORIES: MassIVE