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Upper airway proteomes of infants and children in sub-Saharan Africa

ABSTRACT: Mass spectrometry-based proteomics was carried out on Naso- and oropharyngeal swabs collected from infants and children presenting to hospital with both mild and severe respiratory illnesses as well as age-matched control children who were well and sampled from home.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: James M. Njunge

PROVIDER: MSV000082238 | MassIVE | Wed Apr 04 23:29:00 BST 2018

SECONDARY ACCESSION(S): PXD009403

REPOSITORIES: MassIVE

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Publications

Airway response to respiratory syncytial virus has incidental antibacterial effects.

Sande Charles J CJ Njunge James M JM Mwongeli Ngoi Joyce J Mutunga Martin N MN Chege Timothy T Gicheru Elijah T ET Gardiner Elizabeth M EM Gwela Agnes A Green Christopher A CA Drysdale Simon B SB Berkley James A JA Nokes D James DJ Pollard Andrew J AJ

Nature communications 20190517 1

RSV infection is typically associated with secondary bacterial infection. We hypothesise that the local airway immune response to RSV has incidental antibacterial effects. Using coordinated proteomics and metagenomics analysis we simultaneously analysed the microbiota and proteomes of the upper airway and determined direct antibacterial activity in airway secretions of RSV-infected children. Here, we report that the airway abundance of Streptococcus was higher in samples collected at the time of ...[more]

PMID: 31101811

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