Dataset Information

Crocodile Sperm Proteome

ABSTRACT: In a reproductive strategy that is considered unique to the mammalian lineage, spermatozoa must undergo a series of physiological changes, termed capacitation, in the female reproductive tract prior to developing their capacity to fertilize an ovum. Here, we have employed a comparative proteomic strategy to explore the biological significance of this form of post-testicular maturation in the ancient reptilian species of the Australian saltwater crocodile (Crocodylus porosus).

INSTRUMENT(S): Q Exactive

ORGANISM(S): Crocodylus Porosus (ncbitaxon:8502)

SUBMITTER: Dr. Matt Dun Brett Nixon

PROVIDER: MSV000082258 | MassIVE | Sun Apr 08 23:36:00 BST 2018

REPOSITORIES: MassIVE

ACCESS DATA

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Publications

Modification of Crocodile Spermatozoa Refutes the Tenet That Post-testicular Sperm Maturation Is Restricted To Mammals.

Nixon Brett B Johnston Stephen D SD Skerrett-Byrne David A DA Anderson Amanda L AL Stanger Simone J SJ Bromfield Elizabeth G EG Martin Jacinta H JH Hansbro Philip M PM Dun Matthew D MD

Molecular & cellular proteomics : MCP 20180802 Suppl 1

Competition to achieve paternity has contributed to the development of a multitude of elaborate male reproductive strategies. In one of the most well-studied examples, the spermatozoa of all mammalian species must undergo a series of physiological changes, termed capacitation, in the female reproductive tract before realizing their potential to fertilize an ovum. However, the evolutionary origin and adaptive advantage afforded by capacitation remains obscure. Here, we report the use of comparati ...[more]

PMID: 30072580

Similar Datasets

Project description:Male factors account for approximately 40% of all infertility. Conventional semen analysis focus on seminal physicochemical property and spermatozoa morphology. They yield no information concerning the functional competence of the spermatozoa(Petrunkina et al., 2007). Owning to the essential role of proteins in the reproductive process, comprehensive and systematic identification of proteome is critical to gain new insights into spermatogenesis and infertility. Recent advances in mass spectrometry have allowed the identification of hundreds to thousands of protein in spermatozoa(Oliva et al., 2008; Amaral et al., 2014; Codina et al., 2015). In major domestic mammalian species, global spermatozoa proteomic profiles have been described in rodents(Baker et al., 2008a; Baker et al., 2008b) and bovine(Peddinti et al., 2008). Meanwhile, lacking of new protein synthesis in spermatozoa supports the current view that the regulation of sperm maturation is controlled by exogenous proteins(O'Rand et al., 2011) (e.g., during epididymal transit). Among the seminal plasma proteins, The human seminal plasma has been comprehensively described (Pilch and Mann, 2006; Batruch et al., 2011; Milardi et al., 2012; Milardi et al., 2013; Sharma et al., 2013). In domestic animal species, some studies performing a systematic analysis on using high throughput proteomics have been performed(Kelly et al., 2006; Moura et al., 2007; Souza et al., 2012; Druart et al., 2013; Soleilhavoup et al., 2014). Buffalos (Bubalus bubalis) are adapted to hot–humid tropical climatic conditions, but have low reproductive efficiency. The low sperm motility maybe contributed to protein components variation of spermatozoa and seminal plasma. The composition of buffalo spermatozoa and buffalo seminal plasma (BSP) remains unknown. Proteomic study would be beneficial to the elucidation of the roles of sperm and BSP proteins in regulation of maturation, motility and fertilization. As such, the aim of the current study was to extensively characterize the differentially expressed protein of buffalo spermatozoa and seminal plasma using a comparative proteomics.