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Venom and saliva proteomics of Solenodon

ABSTRACT: Top-down and bottom-up protein analysis of venom and saliva of solenodon. Venom and saliva was separated by HPLC and either directly analysis by HR FT MS/MS (Top-down) or further decomplexed by SDS-PAGE followed by in-gel trypsine digestion and HR LC-MS/MS analysis (bottom-up, Venom only). For shotgun bottom-up comparison of venom and saliva proteins, samples were directly reduced, alkylated, digested with trypsin and measured by HPLC-MS/MS. Additional bottom-up analysis was performed from bioactivity guided fractionation experiments.

INSTRUMENT(S): TripleTOF 5600, LTQ Orbitrap XL

ORGANISM(S): Solenodon (ncbitaxon:79804)

SUBMITTER: Jeroen Kool Eivind Undheim Daniel Petras

PROVIDER: MSV000082307 | MassIVE | Tue Apr 24 17:26:00 BST 2018

SECONDARY ACCESSION(S): PXD009593

REPOSITORIES: MassIVE

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