Proteomics

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SamavarchiTehrani_P112_VS4_Viral_BioID_Benchmark_mouse

ABSTRACT: This submission contains the mass spectrometry files for the manuscript by Samavarchi-Tehrani et al. that describes the use of lentiviral delivery of BirA*-tagged transgenes into HeLa, BJ and MEF cells or in comparison to stably integrated transgene in Flp-In T-REx HeLa cell lines. This submission contains the mouse files from a set of 56 raw MS files and associated peak lists and result files, acquired on a TripleTOF 6600 spectrometer (AB SCIEX, Concord, Ontario, Canada) operated in Data Dependent Acquisition mode. Samples were generated by Payman Samavarchi-Tehrani. This massive submission contains the files for the mouse samples. Please see accompanying submission for the human samples. Raw files are deposited here in MassIVE. For questions, please contact Anne-Claude Gingras (gingras@lunenfeld.ca) or Payman Samavarchi-Tehrani (payman@lunenfeld.ca). Table 1 describes the composition of this dataset Table 2 lists all the peptide identification evidence (as per iProphet) Table 3 lists the SAINTexpress interactions Internal reference from the Gingras lab ProHits implementation: Project 112, Export versions VS4 (Viral BioID Benchmark) SAINT Task ID: Flp-HeLa:3596; HeLa:3139; BJ:3140; MEF:3141

INSTRUMENT(S): TripleTOF 6600

ORGANISM(S): Mus Musculus (ncbitaxon:10090)

SUBMITTER: Anne-Claude Gingras  

PROVIDER: MSV000082429 | MassIVE | Fri Jun 01 14:19:00 BST 2018

SECONDARY ACCESSION(S): PXD010008

REPOSITORIES: MassIVE

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Publications

A Versatile Lentiviral Delivery Toolkit for Proximity-dependent Biotinylation in Diverse Cell Types.

Samavarchi-Tehrani Payman P   Abdouni Hala H   Samson Reuben R   Gingras Anne-Claude AC  

Molecular & cellular proteomics : MCP 20180710 11

Proximity-dependent biotinylation strategies have emerged as powerful tools to characterize the subcellular context of proteins in living cells. The popular BioID approach employs an abortive <i>E. coli</i> biotin ligase mutant (R118G; denoted as BirA*), which when fused to a bait protein enables the covalent biotinylation of endogenous proximal polypeptides. This approach has been mainly applied to the study of protein proximity in immortalized mammalian cell lines. To expand the application sp  ...[more]

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