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A cross-linking-aided mass spectrometry workflow reveals extensive intracellular trafficking in time-resolved, signal-dependent EGFR proteome

ABSTRACT: Here we describe a cross-linking-aided MS workflow for isolation and identification of signal-dependent membrane protein interactomes using EGFR as an example. Employing formaldehyde as a cross-linking reagent, we performed immuno-precipitation of endogenous EGFR upon EGF treatment, facilitating the capture of weak and transient interactions in the proximity of the receptor, and analyzed the associated proteins by quantitative mass spectrometry.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Yi Wang

PROVIDER: MSV000082799 | MassIVE | Tue Aug 07 23:52:00 BST 2018

SECONDARY ACCESSION(S): PXD010717

REPOSITORIES: MassIVE

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