Proteomics

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Major Proteomic Changes Associated with Amyloid Induced Biofilm Formation in Pseudomonas aeruginosa PAO1


ABSTRACT: The newly identified functional amyloids in Pseudomonas (Fap) are associated with increased aggregation and biofilm formation in the opportunistic pathogen P. aeruginosa. However, whether this phenomenon can be simply ascribed to the mechanical properties of the amyloid fibrils remains undetermined. To gain a deeper understanding of the Fap mediated biofilm formation, the physiological consequences of Fap expression were investigated using label-free protein quantification. The functional amyloids were found to not solely act as inert structural biofilm components. Their presence induced major changes in the global proteome of the bacterium. These included the lowered abundance of classical virulence factors such as elastase B and the secretion system of alkaline protease A. Amyloid mediated biofilm formation furthermore increased abundance of the alginate and pyoverdine synthesis machinery, turning P. aeruginosa PAO1 into an unexpected mucoid phenotype. The results imply a significant impact of functional amyloids in P. aeruginosa for biofilm formation and chronic infections.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Pseudomonas Aeruginosa Pao1 (ncbitaxon:208964)

SUBMITTER: Per Halkj��r Nielsen  

PROVIDER: MSV000082917 | MassIVE |

SECONDARY ACCESSION(S): PXD001373

REPOSITORIES: MassIVE

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Major proteomic changes associated with amyloid-induced biofilm formation in Pseudomonas aeruginosa PAO1.

Herbst Florian-Alexander FA   Søndergaard Mads T MT   Kjeldal Henrik H   Stensballe Allan A   Nielsen Per H PH   Dueholm Morten S MS  

Journal of proteome research 20141031 1


The newly identified functional amyloids in Pseudomonas (Fap) are associated with increased aggregation and biofilm formation in the opportunistic pathogen P. aeruginosa; however, whether this phenomenon can be simply ascribed to the mechanical properties of the amyloid fibrils remains undetermined. To gain a deeper understanding of the Fap-mediated biofilm formation, the physiological consequences of Fap expression were investigated using label-free protein quantification. The functional amyloi  ...[more]

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