Proteomics

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Proteomics analysis of nuclear envelope, cytoplasmic membrane and nuclear contents fractions isolated from mesenchymal stem cells, adipocytes and myocytes

ABSTRACT: We used shotgun proteomics to analyze three subcellular fractions isolated from mesenchymal stem cells and from differentiated adipocytes and myocytes: nuclear envelopes (NEs), cytoplasmic membranes and nuclear contents. The proteomics data were analyzed by a NSAF-based scoring system to calculate relative protein enrichment in the NE fraction. High NE enrichment scores were obtained for most well-characterized NE proteins. High scores also were seen for a number of new proteins not known to be concentrated at the NE, and we validated that five of these are substantially concentrated at the NE of multiple cell types. Our datasets can be useful for identifying additional NE-concentrated proteins in the future.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Mus Musculus (ncbitaxon:10090)

SUBMITTER: John R. Yates III   Larry Gerace  

PROVIDER: MSV000083166 | MassIVE | Tue Nov 27 09:33:00 GMT 2018

SECONDARY ACCESSION(S): PXD011856

REPOSITORIES: MassIVE

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Publications

Identification of new transmembrane proteins concentrated at the nuclear envelope using organellar proteomics of mesenchymal cells.

Cheng Li-Chun LC   Baboo Sabyasachi S   Lindsay Cory C   Brusman Liza L   Martinez-Bartolomé Salvador S   Tapia Olga O   Zhang Xi X   Yates John R JR   Gerace Larry L  

Nucleus (Austin, Tex.) 20191201 1

The double membrane nuclear envelope (NE), which is contiguous with the ER, contains nuclear pore complexes (NPCs) - the channels for nucleocytoplasmic transport, and the nuclear lamina (NL) - a scaffold for NE and chromatin organization. Since numerous human diseases linked to NE proteins occur in mesenchyme-derived cells, we used proteomics to characterize NE and other subcellular fractions isolated from mesenchymal stem cells and from adipocytes and myocytes. Based on spectral abundance, we c  ...[more]

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