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PU.1 interactome mouse bone marrow derived dendritic cells

ABSTRACT: Mouse bone marrow derived dendritic cells were generated by culturing bone marrow cells at a density of 0.5x10E6 cells/ml in RPMI-1640 supplemented with 5% FCS, 1% Pen/Strep, 5microM 2-mercaptoethanol, 20ng/ml GM-CSF. At day 7 dendritic cells were stimulated or not with 500 ng/ml LPS, and collected at day 10. 2x10E8 cells were used to prepare whole cell extracts and to perform PU.1 immunoprecipitaion with PU.1 antibody (T-21 Santa Cruz). IgG was used as control.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Mus Musculus (ncbitaxon:10090)

SUBMITTER: Laura Gutierrez

PROVIDER: MSV000083176 | MassIVE | Fri Nov 30 05:21:00 GMT 2018

SECONDARY ACCESSION(S): PXD011904

REPOSITORIES: MassIVE

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Publications

Comparison of the PU.1 transcriptional regulome and interactome in human and mouse inflammatory dendritic cells.

Scheenstra Maaike R MR Martínez-Botía Patricia P Acebes-Huerta Andrea A Brouwer Rutger W W RWW Caballero-Sánchez Noemí N Gillemans Nynke N De Bleser Pieter P Nota Benjamin B De Cuyper Iris M IM Salunkhe Vishal V Woltman Andrea M AM van de Laar Lianne L Rijkers Erikjan E Demmers Jeroen A A JAA van IJcken Wilfred F J WFJ Philipsen Sjaak S van den Berg Timo K TK Kuijpers Taco W TW Gutiérrez Laura L

Journal of leukocyte biology 20201202

Dendritic cells (DCs) are key immune modulators and are able to mount immune responses or tolerance. DC differentiation and activation imply a plethora of molecular and cellular responses, including transcriptional changes. PU.1 is a highly expressed transcription factor in DCs and coordinates relevant aspects of DC biology. Due to their role as immune regulators, DCs pose as a promising immunotherapy tool. However, some of their functional features, such as survival, activation, or migration, a ...[more]

PMID: 33289106

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Expression data of colony-stimulating factor (CSF) grown bone marrow derived cells

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Sumoylation-deficient bone marrow derived dendritic cells transcriptomic analysis after LPS stimulation

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PU1 interactome in human monocyte derived dendritic cells

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Gene expression profiling to identify genes that are differentially expressed between murine MHCIIlo and MHCIIhi dendritic cell subpopulations [MHCIIhi vs. MHCIIlo]

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The role of CD11c in dendritic cells

Project description:We cultured bone marrow derived dendritic cells from WT and CD11c KO mice. Then, a group of bone marrow dendritic cells were stimulated with LPS overnight. We obtained bone marrow derived dendritic cells with or without LPS stimulation and analyzed proteomics profiles.

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Gene expression data from wild-type and Nlrc5 knockout GM-CSF induced bone marrow dendritic cells infected with Newcastle Disease virus.

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