Transient expression of SPS, KOMT1, and CYP719A26 from kava
Ontology highlight
ABSTRACT:
INSTRUMENT(S): Q Exactive
ORGANISM(S): Nicotiana Benthamiana
SUBMITTER: 
Jing-Ke Weng
PROVIDER: MSV000083275 | MassIVE | Sat Dec 22 06:39:00 GMT 2018
REPOSITORIES: MassIVE
Dataset's files
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|---|---|---|---|---|
| MSV000083275 | Other |
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Project description:Transient expression of SPS, KOMT1, and CYP719A26 from kava (Piper methysticum)
2018-12-22 | MSV000083275 | GNPS
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Project description:In the present study, we used the single prolonged stress (SPS) model to mimic posttraumatic stress disorder. High-throughput sequencing were utilized to analyze differentially expressed genes (DEGs) in the hippocampus of control and SPS rats. RNA-seq analysis revealed 230 significantly DEGs between the control and SPS groups.
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Project description:The purpose of this experiment was to assess the effect of a synergistic combination of natural pyrethrin and an ethylacetate extract of Piper nigrum seeds (a botanical insecticide). This effect was compared to the effects of P. nigrum or pyrethrin used alone. Due to the synergistic nature of the mixture, it was predicted that gene expression profiles in this treatment would reflect this effect. Keywords: insecticide response, stress-response
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Project description:Severe angiopathy is a major driver for diabetes associated secondary complications. Knowledge on underlying mechanisms essential for advanced therapies to attenuate these pathologies is limited. Injection of ABCB5+ stromal precursors (SPs) at the edge of non-healing diabetic wounds in a murine db/db model, closely mirroring human type II diabetes, profoundly accelerates wound closure. Strikingly, enhanced angiogenesis was substantially enforced by the release of the ribonuclease angiogenin from ABCB5+ SPs. This compensates for the profoundly reduced angiogenin expression in non-treated murine and human chronic diabetic wounds. Silencing of angiogenin in ABCB5+ SPs prior to injection significantly reduced angiogenesis, reduced numbers of M2 macrophages and delayed wound closure in diabetic db/db mice implying an unprecedented key role for angiogenin in tissue regeneration in diabetes. These data hold significant promise for further refining SPs-based therapies of non-healing diabetic foot ulcers and other pathologies with impaired angiogenesis.
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Project description:This experiment was used to determine the effect of a botanical insecticide upon gene expression profiles in Drosophila melanogaster. Adult female Drosophila (oregon-R strain) were treated with an ethylacetate extract of Piper nigrum (Piperaceae) seeds formulated in 99% ethanol. Treatment was topical, using a Potter's tower to administer a total of 2 mL of a 0.9mg/mL concentration. Control treatment was identical except flies were treated with 99% ethanol as a solvent control. Gene expression was studied four hours post-treatment. Keywords: insecticide response, stress-response
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