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Aqueous humor: control vs naive vs persistent

ABSTRACT: 1234567890 1234567890 1234567890 1234567890 1234567890 1234567890 1234567890

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Wagner Fontes

PROVIDER: MSV000083731 | MassIVE | Sun Apr 28 12:25:00 BST 2019

SECONDARY ACCESSION(S): PXD013654

REPOSITORIES: MassIVE

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Publications

Novel Possible Protein Targets in Neovascular Age-Related Macular Degeneration: A Pilot Study Experiment.

Coronado Bruno Nobre Lins BNL da Cunha Felipe Bruno Santos FBS de Oliveira Raphaela Menezes RM Nóbrega Otávio de Toledo OT Ricart Carlos André Ornelas CAO Fontes Wagner W de Sousa Marcelo Valle MV de Ávila Marcos Pereira MP Martins Aline Maria Araújo AMA

Frontiers in medicine 20220127

Age-related macular degeneration (AMD) is among the world's leading causes of blindness. In its neovascular form (nAMD), around 25% of patients present further anatomical and visual deterioration due to persistence of neovascular activity, despite gold-standard treatment protocols using intravitreal anti-VEGF medications. Thus, to comprehend, the molecular pathways that drive choroidal neoangiogenesis, associated with the vascular endothelial growth factor (VEGF), are important steps to elucidat ...[more]

PMID: 35155457

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Phospholipid profiles of control and glaucomatous human aqueous humor.

Project description:To compare phospholipid (phosphatidylcholine, phosphatidylserine, phosphatidylethanolamine and phosphatidylinositol) profiles of human control and glaucomatous aqueous humor (AQH). AQH samples were procured during surgery from human POAG and control subjects (n = 15 each). Samples were used following institutional review board approved protocols and adhering to the tenets of the Declaration of Helsinki. Lipid extraction was performed using a modification of the Bligh and Dyer method, protein concentrations were determined using the Bradford's method, and select samples were confirmed with Densitometry of PHAST gels. Lipids were identified and subjected to ratiometric quantification using a TSQ Quantum Access Max triple quadrupole mass spectrometer utilizing precursor ion scan (PIS) or neutral ion loss scan (NLS) using appropriate class specific lipid standards in a two step quantification process. The comparative profiles of phosphatidylcholines, phosphatidylserines, phosphatidylethanolamines and phosphatidylinositols between control and glaucomatous AQH showed several species common between them. A number of unique lipids in all four phospholipid classes were also identified in control eyes that were absent in glaucomatous eyes and vice versa. A number of phospholipids were found to be uniquely present in control, but absent in glaucomatous AQH and vice versa. Compared with a previous study of control and POAG red blood cells, a number of these phospholipids are absent locally (AQH).

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Effect of prostaglandin analogs on aqueous humor outflow

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Retinal vascular changes and aqueous humor cytokines changes after aflibercept intravitreal injection in treatment-naive myopic choroidal neovascularization.

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Proteome analysis of human aqueous humor.

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