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Comparison of proteomic profiles from fresh and paraformaldehyde-fixed rat brain cells

ABSTRACT: Protein spectra for fresh and paraformaldehyde (PFA)-fixed rat brain cell suspensions. The purpose of this experiment was to determine if/what differences exist when characterizing proteomic signatures from fresh (Fr) or fixed (PFA) samples. Brain cells were isolated from a single rat and were split into 6 replicates in which half were fixed and half remained fresh (N = 3, n = 5).

INSTRUMENT(S): Q-Exactive Plus (Thermo Fisher Scientific) LC-MS/MS

ORGANISM(S): Rattus Sp. (ncbitaxon:10118)

SUBMITTER: Eric Darling

PROVIDER: MSV000083746 | MassIVE | Mon May 06 11:06:00 BST 2019

REPOSITORIES: MassIVE

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Publications

Generating Cell Type-Specific Protein Signatures from Non-symptomatic and Diseased Tissues.

Sadick Jessica S JS Crawford Lorin A LA Cramer Harry C HC Franck Christian C Liddelow Shane A SA Darling Eric M EM

Annals of biomedical engineering 20200417 8

Here we demonstrate a technique to generate proteomic signatures of specific cell types within heterogeneous populations. While our method is broadly applicable across biological systems, we have limited the current work to study neural cell types isolated from human, post-mortem Alzheimer's disease (AD) and aged-matched non-symptomatic (NS) brains. Motivating the need for this tool, we conducted an initial meta-analysis of current, human AD proteomics studies. While the results broadly corrobor ...[more]

PMID: 32303872

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