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Astrocyte and neuron protein signatures in Alzheimer's disease

ABSTRACT: Protein from astrocytes (glial fibrillary acidic protein-positive cells), neurons (beta-III tubulin-positive cells), and unsorted (cell suspension without any enrichment based on astrocyte or neuron markers) from human, post-mortem Alzheimer's disease (AD) and aged-matched non-symptomatic (NS) prefrontal cortex brain samples.

INSTRUMENT(S): Q-Exactive Plus (Thermo Fisher Scientific) LC-MS/MS

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Eric Darling

PROVIDER: MSV000083747 | MassIVE | Mon May 06 11:38:00 BST 2019

REPOSITORIES: MassIVE

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Publications

Generating Cell Type-Specific Protein Signatures from Non-symptomatic and Diseased Tissues.

Sadick Jessica S JS Crawford Lorin A LA Cramer Harry C HC Franck Christian C Liddelow Shane A SA Darling Eric M EM

Annals of biomedical engineering 20200417 8

Here we demonstrate a technique to generate proteomic signatures of specific cell types within heterogeneous populations. While our method is broadly applicable across biological systems, we have limited the current work to study neural cell types isolated from human, post-mortem Alzheimer's disease (AD) and aged-matched non-symptomatic (NS) brains. Motivating the need for this tool, we conducted an initial meta-analysis of current, human AD proteomics studies. While the results broadly corrobor ...[more]

PMID: 32303872

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