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SETD1A protects from senescence through regulation of the mitotic gene expression program

ABSTRACT: SETD1A, a Set1/COMPASS family member maintaining histone-H3-lysine-4 (H3K4) methylation on transcriptionally active promoters, is overexpressed in breast cancer. Here we show that SETD1Asupports mitotic processes and consequentially, its knockdown induces senescence, independent of mutations in p53, RBand p16, known senescence mediators. The following labeling scheme was used: bridge_1; TMT1_126 A549_GFPctrl_Day3_Repl1; TMT1_127n A549_shSETD1A#1_Day3_Repl1; TMT1_127c A549_shSETD1A#2_Day3_Repl1; TMT1_128n A549_GFPctrl_Day5_Repl1; TMT1_128c A549_shSETD1A#1_Day5_Repl1; TMT1_129n A549_shSETD1A#2_Day5_Repl1; TMT1_129c M231_GFPctrl_Day3; TMT1_130n M231_GFP_ctrl_Day5; TMT1_130c bridge_2; TMT1_131 bridge_1; TMT2_126 A549_GFP_ctrl_Day3_Repl2; TMT2_127n A549_shSETD1A#1_Day3_Repl2; TMT2_127c A549_shSETD1A#2_Day3_Repl2; TMT2_128n A549_GFPctrl_Day5_Repl2; TMT2_128c A549_shSETD1A#1_Day5_Repl2; TMT2_129n A549_shSETD1A#2_Day5_Repl2; TMT2_129c M231_shSETD1A#2_Day3; TMT2_130n M231_shSETD1A#2_Day5; TMT2_130c bridge_2; TMT2_131

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Shyamala Maheswaran

PROVIDER: MSV000083763 | MassIVE | Thu May 09 18:35:00 BST 2019

REPOSITORIES: MassIVE

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SETD1A protects from senescence through regulation of the mitotic gene expression program

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