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Mouse Bone Marrow Proteome Profiling

ABSTRACT: Hematopoietic stem cells give rise to all blood lineages, can fully re-populate the bone marrow, and easily outlive the host organism. To better understand how stem cells remain fit during aging, we analyzed the proteome of hematopoietic stem and progenitor cells.

INSTRUMENT(S): Orbitrap Fusion ETD

ORGANISM(S): Mus Musculus (ncbitaxon:10090)

SUBMITTER: Catic, Andre

PROVIDER: MSV000083845 | MassIVE | Thu May 23 11:44:00 BST 2019

SECONDARY ACCESSION(S): PXD013995

REPOSITORIES: MassIVE

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Publications

Cyclophilin A supports translation of intrinsically disordered proteins and affects haematopoietic stem cell ageing.

Maneix Laure L Iakova Polina P Lee Charles G CG Moree Shannon E SE Lu Xuan X Datar Gandhar K GK Hill Cedric T CT Spooner Eric E King Jordon C K JCK Sykes David B DB Saez Borja B Di Stefano Bruno B Chen Xi X Krause Daniela S DS Sahin Ergun E Tsai Francis T F FTF Goodell Margaret A MA Berk Bradford C BC Scadden David T DT Catic André A

Nature cell biology 20240329 4

Loss of protein function is a driving force of ageing. We have identified peptidyl-prolyl isomerase A (PPIA or cyclophilin A) as a dominant chaperone in haematopoietic stem and progenitor cells. Depletion of PPIA accelerates stem cell ageing. We found that proteins with intrinsically disordered regions (IDRs) are frequent PPIA substrates. IDRs facilitate interactions with other proteins or nucleic acids and can trigger liquid-liquid phase separation. Over 20% of PPIA substrates are involved in t ...[more]

PMID: 38553595

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