Proteomics

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A reference map of the human proinsulin biosynthetic interaction network


ABSTRACT: A monoclonal antibody specific for human proinsulin (20G11) was generated and utilized for affinity purification mass spectrometry (AP-MS) for unbiased profiling of proinsulin protein:protein interactions in human islets. Half of each islet prep was treated with the cytokines IL1-beta and IFN-gamma. Immunoprecipitated proinsulin or control IgG immunoprecipitates from human islets were then subjected to state of the art mass spectrometry revealing a rich proinsulin biosynthetic network that is remarkably conserved across a diverse group of donors.

INSTRUMENT(S): LTQ Orbitrap Velos, Q Exactive

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Pamela Itkin-Ansari  

PROVIDER: MSV000084057 | MassIVE | Wed Jul 03 15:25:00 BST 2019

SECONDARY ACCESSION(S): PXD014476

REPOSITORIES: MassIVE

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