Proteomics

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Blubber proteome response to repeated ACTH administration in juvenile elephant seals


ABSTRACT: Proteins were isolated from the inner blubber layer (closest to muscle) of juvenile elephant seals undergoing a repeated ACTH administration experiment (for details, see McCormley et al., 2018, doi: 10.1093/conphys/coy040; Deyarmin et al., 2019, doi:10.1038/s41598-019-39089-2). Sample preparation was conducted using the Qiazol method as previously described (Khudyakov et al., 2018, doi:10.1242/bio.036731). Four samples of blubber proteins from each of 2 seals (collected before and after the first and fourth ACTH administration) were labeled with 8-plex iTRAQ, fractionated off-line, pooled into 4 fractions, and analyzed by HPLC-MS/MS.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Mirounga Angustirostris (ncbitaxon:9716)

SUBMITTER: Jane Khudyakov  

PROVIDER: MSV000084153 | MassIVE | Mon Jul 29 12:23:00 BST 2019

REPOSITORIES: MassIVE

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Publications

Blubber proteome response to repeated ACTH administration in a wild marine mammal.

Deyarmin Jared J   Hekman Ryan R   Champagne Cory C   McCormley Molly M   Stephan Alicia A   Crocker Daniel D   Houser Dorian D   Khudyakov Jane J  

Comparative biochemistry and physiology. Part D, Genomics & proteomics 20191122


While the response to acute stress is adaptive in nature, repeated or chronic stress can impact an animal's fitness by depleting its energy stores and suppressing immune function and reproduction. This can be especially deleterious for species that rely on energy reserves to fuel key life history stages (e.g. reproduction), already experience physiological extremes (e.g. fasting), and/or have undergone significant population declines, such as many marine mammals. However, identifying chronically  ...[more]

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