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Automated mass spectrometry imaging of over 2000 proteins from tissue sections at 100-um spatial resolution

ABSTRACT: Imaging mass spectrometry is a powerful emerging tool for mapping the spatial distribution of biomolecules across tissue surfaces. Here the authors showcase an automated technology for deep proteome imaging that utilizes ultrasensitive microfluidics and a mass spectrometry workflow to analyze tissue voxels, generating quantitative cell-type-specific images.

INSTRUMENT(S): Q Exactive Plus

ORGANISM(S): Mus Musculus (ncbitaxon:10090)

SUBMITTER: Kristin Burnum-Johnson

PROVIDER: MSV000084421 | MassIVE | Fri Oct 04 13:16:00 BST 2019

REPOSITORIES: MassIVE

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Automated mass spectrometry imaging of over 2000 proteins from tissue sections at 100-μm spatial resolution.

Piehowski Paul D PD Zhu Ying Y Bramer Lisa M LM Stratton Kelly G KG Zhao Rui R Orton Daniel J DJ Moore Ronald J RJ Yuan Jia J Mitchell Hugh D HD Gao Yuqian Y Webb-Robertson Bobbie-Jo M BM Dey Sudhansu K SK Kelly Ryan T RT Burnum-Johnson Kristin E KE

Nature communications 20200107 1

Biological tissues exhibit complex spatial heterogeneity that directs the functions of multicellular organisms. Quantifying protein expression is essential for elucidating processes within complex biological assemblies. Imaging mass spectrometry (IMS) is a powerful emerging tool for mapping the spatial distribution of metabolites and lipids across tissue surfaces, but technical challenges have limited the application of IMS to the analysis of proteomes. Methods for probing the spatial distributi ...[more]

PMID: 31911630

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