Dataset Information

Concentration determination of >200 proteins in dried blood spots for biomarker discovery and validation

ABSTRACT: Raw files were created using XCalibur 3.0.63 (Thermo Scientific) software and analysed with Thermo Proteome Discoverer 2.2.0.388 software suite (Thermo Scientific). The raw data files were processed as previously described 26 with the following modifications: the peak lists were submitted to Mascot 2.4.1and Sequest servers using the UniProt human database 28 which contained 20,338 protein sequences. The enzyme was set to Trypsin (Full) with max missed cleavage set to 2. The fixed modification was set to carbamidomethyl and variable modifications were set as oxidation (M), deamidation (NQ), and N-terminal methionine loss. Precursor and fragment mass tolerances were set at 8 ppm and 0.8 Da, respectively. Peptide spectral match validation was performed using Percolator setting the maximum Delta Cn to 0.05 and decoy database searches at 0.01 for target FDR (Strict), 0.05 (relaxed) and validation based on q-Value

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Christoph H. Borchers

PROVIDER: MSV000084629 | MassIVE | Wed Nov 27 09:57:00 GMT 2019

SECONDARY ACCESSION(S): PXD016484

REPOSITORIES: MassIVE

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Publications

SBRT of Spinal Metastases Using a Simultaneous Integrated Boost Concept in Oligometastatic Cancer Patients Is Safe and Effective.

Waltenberger Maria M Strick Christian C Vogel Marco M E MME Diehl Christian C Combs Stephanie E SE

Cancers 20231212 24

(1) Purpose: To assess the safety and effectivity of stereotactic body radiotherapy (SBRT) on spinal metastases utilizing a simultaneous integrated boost (SIB) concept in oligometastatic cancer patients. (2) Methods: 62 consecutive patients with 71 spinal metastases received SIB-SBRT between 01/2013 and 09/2022 at our institution. We retrospectively analyzed toxicity, local tumor control (LC), and progression-free (PFS) and overall survival (OS) following SIB-SBRT and assessed possible influenci ...[more]

PMID: 38136357

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