Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Halo-C11orf84_transient-transfection_Halo-AP-MudPIT

ABSTRACT: Transient transfection 293FRT cells Halo-C11orf84 and Halo-SNAP control Halo Purification Lys-C;Trypsine LTQ-MudPIT

INSTRUMENT(S): LTQ

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Michael P. Washburn

PROVIDER: MSV000084678 | MassIVE | Wed Dec 11 15:48:00 GMT 2019

REPOSITORIES: MassIVE

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Halo-SPIN1_stable-cell-line_Halo-AP-MudPIT

Project description:Halo-SPIN1 stable cell line clone #9 & #11 in 293FRT cells Blank 293FRT control Halo purification Lys-C;Trypsin LTQ MudPIT

2019-12-11 | MSV000084679 | MassIVE

Halo-SPIN1/SNAP-SPINDOC SNAP-Halo-SCAP E1/UB2/E2-MudPIT

Project description:Halo-SPIN1 SNAP-SPINDOC Seriel capture affinity purified E1;UB2;E2 MudPIT

2019-12-17 | MSV000084713 | MassIVE

WDR76-SPIN1_inWDR76KO_SCAP-MS_MudPIT

Project description:Serial Capture Affinity Purification (SCAP) samples analyzed using Multidimentional Protein identification Technology (MudPIT) on Orbitrap Velos Elite. Baits are SNAP-SPIN1 and Halo-WDR76. SNAP purification was performed first, then Halo purification was performed. SCAP was performed using whole cell extract from a stable cell line of 293FRT cells in WDR76 KO background. 3 biological replicates of E1, UB2 and E2 fractions.

2021-02-03 | MSV000086802 | MassIVE

Halo-WDR76_Purification_WDR76KObackground_MudPIT_wPTMs

Project description:Halo purification samples analyzed using Multidimentional Protein identification Technology (MudPIT) on Orbitrap Velos Elite. Baits Halo-WDR76. Purification was performed using whole cell extract from a stable cell line of 293FRT cells in WDR76 KO background. Control purification was performed on blank WDR76 KO293FRT cells. 3 biological replicates of control and Halo-WDR76.

2021-02-11 | MSV000086849 | MassIVE

Halo-Control/WDR76/SPIN1/CBX5_purification_MudPIT_wPTMs

Project description:Halo purification samples analyzed using Multidimentional Protein identification Technology (MudPIT) on Orbitrap Velos Elite. Baits are Halo-WDR76, Halo-SPIN1, Halo-CBX5. The bait for Control Purification is Halo tag only. Purification for each bait was performed using whole cell extract from a stable cell line in 293FRT cells. 3 biological replicates for each type of bait.

2021-02-11 | MSV000086846 | MassIVE

Analysis of subcellular transcriptomes by RNA proximity labeling with Halo-seq

Project description:We have developed Halo-seq, an RNA proximity labeling method that allows the quantification of subcellular transcriptomes. We have demonstrated the efficacy of Halo-seq here by using it to quantify chromatin-proximal, nucleolar, and cytoplasmic transcriptomes. In Halo-seq, RNA molecules in close proximity to a spatially restricted protein are specifically marked and biotinylated, facilitating their separation from bulk cellular RNA and their quantification.

2021-04-19 | GSE172281 | GEO

halo-alkaliphilic mixed bacterial consortium

Project description:halo-alkaliphilic mixed bacterial consortium Metagenome

| PRJNA628135 | ENA

Halo-SPIN1/SNAP-SPINDOC SNAP-Halo-SCAP E2-DSSO-XL

Project description:Halo-SPIN1 SNAP-SPINDOC Seriel Capture Affinity Purified E2; on-beads DSSO crosslinking ms1/ms2/ms3 PD export (combined)

2019-12-18 | MSV000084719 | MassIVE

Genome-wide binding profile of Halo-tagged cohesin

Project description:We determined the genome-wide binding profile of CTCF and cohesin subunits Smc1a and Smc3 in wild type mouse ES cells and compared them to a double FLAG-Halo-mCTCF/mRad21-SNAPf-V5 knock-in mES line, generated by CRISPR/Cas9-mediated genome editing to perform imaging experiments.

2024-02-01 | GSE104000 | GEO

Halo-WDR76_Purification_inWDR76KO_DSSO-XL-MS

Project description:Halo purified WDR76 from a stable cell line of 293FRT in WDR76 KO background. DSSO crosslinking performed in eluted samples. Data collected using ms1/ms2/ms3 method. Data searched using Proteomics Discoverer 2.4 with XlinkX.

2021-02-09 | MSV000086828 | MassIVE

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data