Proteomics

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Endophyte-promoted Phosphorous Fixation in Poplar

ABSTRACT: Studying the endophyte strains of poplar that help the plant solubilize phosphate, we observed direct evidence of endophyte-promoted phosphorous fixation. Using synchrotron x-ray fluorescence (SR-XRF) microscopy combined with x-ray absorption near-edge structure (XANES), we visualized the nutrient phosphorous inside poplar roots inoculated by the selected endophytes and found that the phosphorus is fixated in the form of organic phosphate inside the root. Proteomics characterization on poplar roots revealed novel proteins and metabolic pathways involved in endophyte enriched phosphorus uptake.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Populus Trichocarpa (ncbitaxon:3694)

SUBMITTER: Sharon Lafferty Doty  

PROVIDER: MSV000084860 | MassIVE | Wed Jan 29 19:46:00 GMT 2020

SECONDARY ACCESSION(S): PXD017325

REPOSITORIES: MassIVE

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Phosphorus is one of the essential nutrients for plant growth, but it may be relatively unavailable to plants because of its chemistry. In soil, the majority of phosphorus is present in the form of a phosphate, usually as metal complexes making it bound to minerals or organic matter. Therefore, inorganic phosphate solubilization is an important process of plant growth promotion by plant associated bacteria and fungi. Non-nodulating plant species have been shown to thrive in low-nutrient environm  ...[more]

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