Maximizing Confidence and Sensitivity in quantitative Phosphoproteomics - Targets of Mec1 kinase
Ontology highlight
ABSTRACT:
INSTRUMENT(S): Q Exactive HF
ORGANISM(S): Saccharomyces Cerevisiae (ncbitaxon:4932)
SUBMITTER: 
Marcus Smolka
PROVIDER: MSV000084875 | MassIVE | Thu Jan 30 18:19:00 GMT 2020
SECONDARY ACCESSION(S): PXD017339
REPOSITORIES: MassIVE
Publications
Maximized quantitative phosphoproteomics allows high confidence dissection of the DNA damage signaling network.
Faca Vitor Marcel VM Sanford Ethan J EJ Tieu Jennifer J Comstock William W Gupta Shagun S Marshall Shannon S Yu Haiyuan H Smolka Marcus B MB
Scientific reports 20201022 1
The maintenance of genomic stability relies on DNA damage sensor kinases that detect DNA lesions and phosphorylate an extensive network of substrates. The Mec1/ATR kinase is one of the primary sensor kinases responsible for orchestrating DNA damage responses. Despite the importance of Mec1/ATR, the current network of its identified substrates remains incomplete due, in part, to limitations in mass spectrometry-based quantitative phosphoproteomics. Phosphoproteomics suffers from lack of redundanc ...[more]
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