Proteomics

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Comaprison of the phagosome proteome of bone marrow-derived macrophages and RAW264.7

ABSTRACT: Macrophages are important immune cells operating at the forefront of innate immunity by taking up foreign particles and microbes through phagocytosis. The RAW 264.7 cell line is commonly used for experiments in the macrophage and phagocytosis field. However, little is known how its functions compare to primary macrophages. Here, we have performed an in-depth proteomics characterisation of phagosomes from RAW 264.7 and bone marrow-derived macrophages by quantifying more than 2500 phagosomal proteins. Our data indicates that there are significant differences for a large number of proteins including important receptors such as mannose receptor 1 and Siglec-1.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Mus Musculus (ncbitaxon:10090)

SUBMITTER: Matthias Trost  

PROVIDER: MSV000084938 | MassIVE | Sat Feb 08 00:58:00 GMT 2020

SECONDARY ACCESSION(S): PXD001293

REPOSITORIES: MassIVE

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High-resolution quantitative proteome analysis reveals substantial differences between phagosomes of RAW 264.7 and bone marrow derived macrophages.

Guo Manman M   Härtlova Anetta A   Dill Brian D BD   Prescott Alan R AR   Gierliński Marek M   Trost Matthias M  

Proteomics 20150205 18

Macrophages are important immune cells operating at the forefront of innate immunity by taking up foreign particles and microbes through phagocytosis. The RAW 264.7 cell line is commonly used for experiments in the macrophage and phagocytosis field. However, little is known how its functions compare to primary macrophages. Here, we have performed an in-depth proteomics characterization of phagosomes from RAW 264.7 and bone marrow derived macrophages by quantifying more than 2500 phagosomal prote  ...[more]