Project description:CHOPPER (Chemical enrichment of protease site with purchasable, elutable reagents, an N terminomics method) with DMSO-treated Jurkat cells
Project description:CHOPPER (Chemical enrichment of protease sites with purchasable, elutable reagents, an N terminomics method) with etoposide-treated Jurkat cells
Project description:The LEDGF transcript from the PSIP1 gene was knocked down in Jurkat cells using RNAi technology. The resulting Jurkat-derived cell line (Jurkat-siJK2) was compared to a control cell line (wild type Jurkat) using microarray analysis. Genes identified as being modulated by LEDGF were preferential targets of HIV integration. Keywords: effects of gene knockdown
Project description:Jurkat T cell line was transfected with SIV Nef controlled by an unducible promoter system. Nef was induced by addition of 10 micromolar pronasterone A and gene expression values were determined after 24 hrs. Control Jurkat cells were untransfected and treated with 10 micromolar pronasterone A and gene expressio values were determined after 24 hrs. Keywords: SIV Nef, Jurkat, pronasterone A