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Spatial Tumor Proteome Profiling by Immunohistochemistry-Based Laser Capture Microdissection and Data-Independent Proteomics

ABSTRACT: We designed an immunohistochemistry (IHC)-based workflow for cell type-resolved proteome analysis of tissue samples. Firstly, targeted cell type was marked by IHC using antibody targeting cell-type specific marker to improve accuracy and efficiency of LCM. Secondly, to increase protein recovery from chemically crosslinked IHC tissues, we optimized a decrosslinking procedure to seamlessly combine with the integrated spintip-based sample preparation technology SISPROT. This newly developed approach, termed IHC-SISPROT, has comparable performance as H&E staining-based proteomic analysis.

INSTRUMENT(S): Q Exactive HF-X

ORGANISM(S): 9606

SUBMITTER: Ruijun Tian

PROVIDER: MSV000085131 | MassIVE | Fri Mar 20 20:38:00 GMT 2020

REPOSITORIES: MassIVE

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