Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Lipidomics of dendritic cells treated with adjuvant

ABSTRACT: Discovery lipidomics was performed on media (+/- Adjuvant) and cultured dendritic cells (+/- Adjuvant or LPS). Samples were from two growth batches, and in each batch there were n=3 for each cell condition and n=1 for media samples.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Mus Musculus (ncbitaxon:10090)

SUBMITTER: Joshua Coon

PROVIDER: MSV000085222 | MassIVE | Thu Apr 02 12:58:00 BST 2020

REPOSITORIES: MassIVE

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

GNPS - Lipidomics of dendritic cells treated with adjuvant

Project description:Discovery lipidomics was performed on media (+/- Adjuvant) and cultured dendritic cells (+/- Adjuvant or LPS). Samples were from two growth batches, and in each batch there were n=3 for each cell condition and n=1 for media samples.

2020-04-02 | MSV000085222 | GNPS

The adjuvant combination of dmLT and MPL-A activates the canonical, non-pyroptotic NLRP3 inflammasome in dendritic cells

Project description:The purpose if this experiment was to determine how a 24 hour treatment with dmLT, MPL-A, and the combination thereof induced gene expression in dendritic cells when compared to LPS and ATP treatment. The knowledge gained from this study would allow us to identify mechanistic pathways that are unique to each adjuvant, which would allow us to further explore their function in DCs.

2023-03-15 | GSE227294 | GEO

The role of CD11c in dendritic cells

Project description:We cultured bone marrow derived dendritic cells from WT and CD11c KO mice. Then, a group of bone marrow dendritic cells were stimulated with LPS overnight. We obtained bone marrow derived dendritic cells with or without LPS stimulation and analyzed proteomics profiles.

2024-01-26 | PXD048907 |

Gene expression profiling of dendritic cells in different physiological stages under Cordyceps sinensis treatment

Project description:Cordyceps sinensis (CS) has been commonly used as a herbal medicine as well as a health supplement in China for over two thousand years. Although previous studies demonstrated that CS has benefit in immunoregulation and anti-inflammation, the precise mechanism by which CS affects immunomodulation is still unclear. In this study, we adopted duplicate sets of loop-design microarray experiments to examine two different batches of CS and analyzed the effects of CS on dendritic cells (DCs) in different physiology stages, naïve stage and inflammatory stage. We used duplicate sets of loop-design microarray in this study. Each loop experiment consisted of 4 RNA samples, including untreated samples, CS 1μg/ml, LPS 1μg/ml, and LPS 1μg/ml+ CS 1μg/ml.

2011-09-08 | E-GEOD-24191 | biostudies-arrayexpress

Effect of W805EC nanoemulsion (NE) on global gene transcription in JawsII dendritic cells in vitro

2013-11-09 | GSE51804 | GEO

Expression data from mouse dendritic cells

Project description:Dendritic cells (DCs) are pivotal for both recognition of antigens and control of an array of immune responses by recognizing microbes through distinct pattern recognition receptors (PRRs). The first microbial component to be studied in detail and known to cause septic shock is endotoxin (LPS). DCs recognize LPS via Toll-like receptor TLR-47. LPS causes many changes in the DCs, but the elicitation of cytokine production is perhaps the one with clear biologic relevance. We used microarrays to detail the global programme of gene expression underlying regulation of TLR 4 signaling and identified the upregulated and downregulated genes in response to LPS treatment in mouse dendritic cells. We use microarray to determine the conserved hematopoietic miRNAs to study their potential contribution to regulating many different immunological cellular processes and contexts. To obtain overall gene expression profile, we extracted bone marrow cells from at least 3 mice for each experiment. Purified ( 97~98% purity) mouse dendritic cells, treated with LPS or control diluent, were used for RNA extraction and hybridization on Affymetrix microarrays. To obtain overall miRNA expression profile, we extracted bone marrow cells from at least 3 mice (C57BL/6) for each experiment. Purified ( 97~98% purity for CD11C+ ) mouse dendritic cells were used for RNA extraction and hybridization on Exiqon miRCURY LNATM microRNA array.

2011-04-05 | E-GEOD-28340 | biostudies-arrayexpress

Tolerogenic dendritic cells protect against acute kidney injury

Project description:We use tolDCs from syngeneic (C57BL6) and allogeneic (BALB/c) mice to show potent renoprotective abilities in a ischemia reperfusion AKI model. Dendritic cells were derived from mice bone marrow and cultured for 7 days with GM-CSF and IL-4. Tolerogenic DCs were cultured in the same DC media but with the addition of VitD3 and IL10. LPS was added on day 6 and cells harvested for use or analysis on day 7.

2023-07-01 | GSE205322 | GEO

Monocyte Derived Dendritic Cell Maturation

Project description:Human monocyte derived dendritic cells matured via galectin-1 or LPS. Keywords: dendritic cell maturation

2006-08-02 | GSE4984 | GEO

RNA-seq analysis of HeLa cells in nutrient-rich and nutrient-deprived media

Project description:RNA-seq analysis was applied in two independent batches, each one in triplicate samples of HeLa cells in nutrient-rich and nutrient-deprived media

2023-04-05 | GSE211066 | GEO

Effect of W805EC nanoemulsion (NE) on global gene transcription in JawsII dendritic cells in vitro

Project description:Nanoemulsion adjuvant affects immune gene expression in dendritic cells. Microarray was used to asses global changes in gene expression in JAWS II dendritic cells upon treatment with nanoemulsion (NE) adjuvant W805EC JawsII cell were incubated with NE formulation for 6 and 24 hours, and RNA was extracted for Affymetrix hybridization

2013-11-09 | E-GEOD-51804 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data