Dataset Information

Defective insulin receptor signaling in hPSCs skews pluripotency and negatively perturbs neural differentiation

ABSTRACT: Proteomics and phosphoproteomics analyses of two human pluripotent stem cell (hPSC) lines with stable insulin receptor knock down. 6-plex TMT-based strategy was applied to compare control versus insulin receptor (IR) knockdown (n=3 replicates for each condition) for each cell lines (CHB8 and H9). Data was searched with MS-GF+ using PNNL's DMS Processing pipeline.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Wei-Jun Qian

PROVIDER: MSV000085298 | MassIVE | Thu Apr 16 18:10:00 BST 2020

SECONDARY ACCESSION(S): PXD018606

REPOSITORIES: MassIVE

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Defective insulin receptor signaling in hPSCs skews pluripotency and negatively perturbs neural differentiation.

Teo Adrian Kee Keong AKK Nguyen Linh L Gupta Manoj K MK Lau Hwee Hui HH Loo Larry Sai Weng LSW Jackson Nicholas N Lim Chang Siang CS Mallard William W Gritsenko Marina A MA Rinn John L JL Smith Richard D RD Qian Wei-Jun WJ Kulkarni Rohit N RN

The Journal of biological chemistry 20210101

Human embryonic stem cells are a type of pluripotent stem cells (hPSCs) that are used to investigate their differentiation into diverse mature cell types for molecular studies. The mechanisms underlying insulin receptor (IR)-mediated signaling in the maintenance of human pluripotent stem cell (hPSC) identity and cell fate specification are not fully understood. Here, we used two independent shRNAs to stably knock down IRs in two hPSC lines that represent pluripotent stem cells and explored the c ...[more]

PMID: 33667549

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