Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

TBL1XR1 MUTATIONS DRIVE EXTRANODAL LYMPHOMA BY INDUCING A PRO-TUMORIGENIC MEMORY FATE

ABSTRACT: Data contains LC-MS data of proximity-based biotin labeling assay (BioID) to generate the protein interaction network of TBL1XR1 (WT and Y446S mutant) protein fused with to FlagBirA (N-terminal tag) and expressed in U2932 and OCILY1 cells.

INSTRUMENT(S): Q-Exactive HF (Thermo Scientific)

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Brian Raught

PROVIDER: MSV000085310 | MassIVE | Mon Apr 20 06:53:00 BST 2020

SECONDARY ACCESSION(S): PXD018676

REPOSITORIES: MassIVE

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

TBL1XR1 Mutations Drive Extranodal Lymphoma by Inducing a Pro-tumorigenic Memory Fate.

Project description:The most aggressive B cell lymphomas frequently manifest extranodal distribution and carry somatic mutations in the poorly characterized gene TBL1XR1. Here, we show that TBL1XR1 mutations skew the humoral immune response toward generating abnormal immature memory B cells (MB), while impairing plasma cell differentiation. At the molecular level, TBL1XR1 mutants co-opt SMRT/HDAC3 repressor complexes toward binding the MB cell transcription factor (TF) BACH2 at the expense of the germinal center (GC) TF BCL6, leading to pre-memory transcriptional reprogramming and cell-fate bias. Upon antigen recall, TBL1XR1 mutant MB cells fail to differentiate into plasma cells and instead preferentially reenter new GC reactions, providing evidence for a cyclic reentry lymphomagenesis mechanism. Ultimately, TBL1XR1 alterations lead to a striking extranodal immunoblastic lymphoma phenotype that mimics the human disease. Both human and murine lymphomas feature expanded MB-like cell populations, consistent with a MB-cell origin and delineating an unforeseen pathway for malignant transformation of the immune system.

| S-EPMC7384961 | biostudies-literature

TBL1XR1 mutations bias germinal center B-cells towards a pro-tumorigenic memory fate

Project description:Diffuse large B-cell lymphomas (DLBCL) encompass a heterogeneous group of diseases derived from different stages of B-cell differentiation. The most clinically aggressive forms of DLBCL, C5/MCD, carry activating mutations in canonical B-cell signaling pathways, as well as early-occurring hits in the poorly-characterized gene TBL1XR1. Here, we investigated the role of TBL1XR1 mutations in the early steps of malignant transformation. We found that TBL1XR1 mutations disrupt germinal center development, and introduce a cell fate bias towards memory B (MB) cells. At the molecular level, TBL1XR1 mutations trigger a switch, by which the SMRT/HDAC3 co-repressor complex shuttles between the lineage-defining transcription factors BCL6 and BACH2. We further demonstrate that terminal differentiation of TBL1XR1 mutant MB is limited upon recall, and that these MB re-enter the germinal center reaction instead, suggesting a role for these cells as the cell-of-origin of C5/MCD DLBCL.

2020-07-02 | GSE139059 | GEO

USP7 Regulates Cytokinesis through FBXO38 and KIF20B

Project description:Data contains LC-MS data of proximity-based biotin labeling assay (BioID) to generate the protein interaction network of FbxO38 protein fused with FlagBirA (N-terminal tag) and expressed in T-REx HEK293 cells.

2018-06-07 | MSV000082444 | MassIVE

Spatial and proteomic profiling reveals centrosome-independent features of centriolar satellites

Project description:Data contains LC-MS data of proximity-based biotin labeling assay (BioID) to generate the protein interaction network of centriolar satellite proteins fused with FlagBirA (N-terminal tag) and expressed in T-REx HEK293 cells.

2018-11-08 | MSV000083121 | MassIVE

Biological roles of SARS-CoV-2 proteins

Project description:Data contains LC-MS data of proximity-based biotin labeling assay (BioID) to generate the protein interaction network of SARS-CoV-2 proteins fused to FlagBirA (N-terminal tag) expressed in T-REx HEK293 cells.

2020-08-24 | MSV000086006 | MassIVE

Atg16L1 deficiency induces insulin resistance through KLHL9-KLHL13-CUL3 complex-mediated IRS1 degradation

Project description:Data contains LC-MS data of proximity-based biotin labeling assay (BioID) to generate the protein interaction network of KLHL9 and KLHL13 proteins fused to FlagBirA (N-terminal tag) and expressed in T-REx HEK293 cells.

2019-04-02 | MSV000083653 | MassIVE

BioID performed on Golgi-enriched fractions identify C10orf76 as a GBF1 Binding Protein essential for Golgi maintenance and secretion

Project description:Data contains LC-MS data of proximity-based biotin labeling assay (BioID) to generate the protein interaction network of GBF1 protein fused to FlagBirA (N-terminal tag) and expressed in HeLa cells and extracted from whole cell lysates and Golgi fractions.

2019-05-27 | MSV000083866 | MassIVE

Global interactomics of Zika Virus

Project description:Protein interaction network of Zika viral proteins generated using affinity-purification (AP-MS) and proximity-based biotin labeling (BioID) assays. Viral proteins were expressed in T-Rex HEK293 cells as fused constructs with either FlagBirA (N-terminal tag) or -BirAFlag (C-temrinal tag).

2018-04-26 | MSV000082311 | MassIVE

MYC dephosphorylation by the PP1/PNUTS phosphatase complex regulates chromatin binding and protein stability

Project description:Data contains LC-MS data of proximity-based biotin labeling assay (BioID) to generate the protein interaction network of Myc protein fused with FlagBirA (N-terminal tag) and expressed in HeLa cells. Data contains LC-MS data of phosphopeptide-enriched lysates from MCF10 cells

2018-05-31 | MSV000082424 | MassIVE

NLRP6 controls PTEN stability by promoting autophagic degradation of p85α to drive tumorigenesis

Project description:To identify NLRP6-associated proteins in living cells, we applied an APEX2-based labelling method combined with mass spectrometry. In brief, APEX2 was genetically fused to NLRP6. In the presence of hydrogen peroxide, APEX2 catalysed biotin-phenol to become a biotin-phenol radical that covalently bound to NLRP6 neighbouring proteins (<20 nm). The biotinylated proteins were enriched and collected by streptavidin beads. One-dimensional SDS–PAGE followed by liquid chromatography-tandem mass spectrometry (GeLC–MS/MS) was performed to identify the proteins. To detect ubiquitinated p85α, a Flag-tagged p85α plasmid was transfected into cells. The cell lysates were immunoprecipitated with anti-Flag and separated on a 6% SDS–PAGE gel. After in-gel digestion, the resulting peptides were extracted for GeLC–MS/MS analysis.

2023-07-02 | PXD037436 | Pride

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data