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Middle-up analysis of polyclonal IgG-Fc

ABSTRACT: Middle-up strategy for the analysis of the fragment crystallizable (Fc) region of human plasma immunoglobulins G (IgGs). The use of hydrophilic interaction liquid chromatography (HILIC) and capillary electrophoresis (CE) coupled to mass spectrometry (MS) allowed to get a comprenhesive view of the N-glycosylation and other PTMs for each subclass and allotypes.

INSTRUMENT(S): maXis, impact

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Manfred Wuhrer

PROVIDER: MSV000085388 | MassIVE | Fri May 08 04:57:00 BST 2020

REPOSITORIES: MassIVE

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Publications

MS-Based Allotype-Specific Analysis of Polyclonal IgG-Fc <i>N</i>-Glycosylation.

Sénard Thomas T Gargano Andrea F G AFG Falck David D de Taeye Steven W SW Rispens Theo T Vidarsson Gestur G Wuhrer Manfred M Somsen Govert W GW Domínguez-Vega Elena E

Frontiers in immunology 20200821

Current approaches to study glycosylation of polyclonal human immunoglobulins G (IgG) usually imply protein digestion or glycan release. While these approaches allow in-depth characterization, they also result in a loss of valuable information regarding certain subclasses, allotypes and co-occuring post-translational modifications (PTMs). Unfortunately, the high variability of polyclonal IgGs makes their intact mass spectrometry (MS) analysis extremely challenging. We propose here a middle-up st ...[more]

PMID: 32973813

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