Queen spermathecal fluid proteomics to investigate proteins underlying variation in stored sperm viability
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ABSTRACT: Queens of social insects have the remarkable ability to keep sperm alive for years within their specialized storage organ, the spermatheca. Despite being one of the most critical determinants of colony health, precisely what biochemical processes enable this sustained sperm viability are largely unknown. Here, we survey quality metrics (sperm count, sperm viability, and ovary size) of honey bee queens from nine genetic sources. We then performed quantitative proteomics on spermathecal fluid from N = 123 individual queens in order to investigate the biochemical processes underlying natural variation in sperm viability.
INSTRUMENT(S): Bruker Impact II Q-TOF
ORGANISM(S): Apis Mellifera (ncbitaxon:7460)
SUBMITTER: Leonard Foster
PROVIDER: MSV000085428 | MassIVE | Sat May 16 22:28:00 BST 2020
SECONDARY ACCESSION(S): PXD019235
REPOSITORIES: MassIVE
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