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Using isotopically labeled calibrants to quantify Heparan sulfate

ABSTRACT: This dataset contains the all the mass spectral data related to this research.

INSTRUMENT(S): Thermo Scientific TSQ Fortis

ORGANISM(S): Quantification Analysis Of Hs Using Lc-msms

SUBMITTER: Jian Liu

PROVIDER: MSV000085577 | MassIVE | Thu Jun 11 13:20:00 BST 2020

REPOSITORIES: MassIVE

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Publications

Quantitative analysis of heparan sulfate using isotopically labeled calibrants.

Wang Zhangjie Z Arnold Katelyn K Xu Yongmei Y Pagadala Vijayakanth V Su Guowei G Myatt Hannah H Linhardt Robert J RJ Liu Jian J

Communications biology 20200804 1

Heparan sulfate is a sulfated polysaccharide that displays essential physiological functions. Here, we report a LC-MS/MS-based method for quantitatively determining the individual disaccharide composition and total amount of heparan sulfate. Using eight 13C-labeled disaccharide calibrants and one 13C-labeled polysaccharide calibrant, we complete the analysis in one-pot process. The method is both sensitive and has the throughput to analyze heparan sulfate from mouse tissues ...[more]

PMID: 32753592

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