Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Human NVU - Astrocytes Methamphetamine

ABSTRACT: We used cortical human fetal astrocytes, density 8-15000 cells/cm2 (ScienCell, San Diego, CA, USA seeded separately in Astrocyte medium (ScienCell, San Diego, CA, USA) P0. Methamphetamine (Sigma-Aldrich, St Louis, MO, USA) was prepared at a stock concentration of 10mM in distilled water. Methamphetamine dilutions 50 micromolar were made in Endo and Neuro/Astro/Peri media respectively and applied to the cells for 26 hrs. Further info in "Proteomic and metabolomic characterization of human neurovascular unit cells in response to methamphetamine" Herland et al Adv Biosystems 2020 [doi:10.25345/C55F3P] [dataset license: CC0 1.0 Universal (CC0 1.0)]

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Anna Herland

PROVIDER: MSV000085795 | MassIVE | Tue Jul 21 00:03:00 BST 2020

REPOSITORIES: MassIVE

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

GNPS - Human NVU - Astrocytes Methamphetamine

Project description:We used cortical human fetal astrocytes, density 8-15000 cells/cm2 (ScienCell, San Diego, CA, USA seeded separately in Astrocyte medium (ScienCell, San Diego, CA, USA) P0. Methamphetamine (Sigma-Aldrich, St Louis, MO, USA) was prepared at a stock concentration of 10mM in distilled water. Methamphetamine dilutions 50 micromolar were made in Endo and Neuro/Astro/Peri media respectively and applied to the cells for 26 hrs. Further info in "Proteomic and metabolomic characterization of human neurovascular unit cells in response to methamphetamine" Herland et al Adv Biosystems 2020 [doi:10.25345/C55F3P] [dataset license: CC0 1.0 Universal (CC0 1.0)]

2020-07-21 | MSV000085795 | GNPS

GNPS - Human NVU - Cortical Astrocytes

Project description:Cortical human fetal astrocytes, density 8-15000 cells/cm2 ( ScienCell, San Diego, CA, USA) seeded in Astrocyte medium (ScienCell, San Diego, CA, USA). Further info in "Proteomic and metabolomic characterization of human neurovascular unit cells in response to methamphetamine" Herland et al Adv Biosystems 2020

2020-07-23 | MSV000085817 | GNPS

Human NVU - Endothelial cells - Methamphetamine

Project description:For human brain microvascular endothelial cells, we used adult passage 3 cortical cells (hBMVECs, CellSystems, Kirkland, WA, USA). Cells were seeded at 13-15000 cells/cm2 in CSC complete medium (Cell Systems, Kirkland, WA, USA) on tissue culture plastic culture dishes coated with Attachment factor for 30 min (Cell Systems, Kirkland, WA, USA). Methamphetamine (Sigma-Aldrich, St Louis, MO, USA) was prepared at a stock concentration of 10mM in distilled water. Methamphetamine dilutions 50 micromolar were made in Endo and Neuro/Astro/Peri media respectively and applied to the cells for 26 hrs. Further info in "Proteomic and metabolomic characterization of human neurovascular unit cells in response to methamphetamine" Herland et al Adv Biosystems 2020

2020-07-20 | MSV000085789 | MassIVE

GNPS - Human NVU - Fetal Brain Pericytes -Methamphetamine

Project description:For human brain pericytes, we used fetal cells passage 3 (ScienCell, San Diego, CA, USA ) seeded Pericyte medium (ScienCell, San Diego, CA, USA) at density 8-15000 cells/cm2 tissue culture plastic culture dishes coated with Attachment Factor for 30 min (Cell Systems, Kirkland, WA, USA). Methamphetamine (Sigma-Aldrich, St Louis, MO, USA) was prepared at a stock concentration of 10mM in distilled water. Methamphetamine dilutions 50 micromolar were made in Endo and Neuro/Astro/Peri media respectively and applied to the cells for 26 hrs. Further info in "Proteomic and metabolomic characterization of human neurovascular unit cells in response to methamphetamine" Herland et al Adv Biosystems 2020

2020-07-23 | MSV000085820 | GNPS

GNPS - Human NVU - Fetal Brain Pericytes

2020-07-23 | MSV000085818 | GNPS

GNPS - Human NVU - Endothelial cells - Methamphetamine

2020-07-20 | MSV000085789 | GNPS

Temporal phosphoproteomic analysis of VEGF-A signaling in HUVECs: An insight into early signaling events associated with angiogenesis

Project description:Aim of the study was to elucidate the phosphoproteomics alterations caused by the treatment of VEGF-A in HUVEC cells at early timepoints of 1, 5 and 10 minutes. Label-free quantitative temporal phosphoproteomics experiments were carried out using LC-MS/MS methods. LTQ orbitrap XL ETD (Thermo Electron, San Jose, CA, USA) connected to UltiMate 3000 nanoLC Pump (Dionex Co., Sunnyvale, CA, USA) was used for data acquisition.

2023-10-24 | PXD038133 | Pride

microRNA changes during pDC activation

Project description:To identify microRNA changes during plasmacytoid dendritic cell (PDC) activation, we stimulated human primary PDCs with 10ug/ml R837 (Invivogen, San Diego, CA, USA) for 4 hours.

2013-03-25 | GSE21160 | GEO

Expression profile of HCT116 cells transfected with miR-204-5p

Project description:We have employed whole genome microarray expression profiling as a discovery platform to screen potential target genes of miR-204-5p in colorectal cancer cell HCT116. HCT116 cells were seeded in 6-cm2 tissue culture plates and transfected with the miR-204-5p mimic or negative control (NC) using Lipofectamine 2000 (Invitrogen, USA). After propagation for 48 hours, total RNA was extracted using TRIzol reagent (Invitrogen, USA). Expression profiling was performed using an Agilent human whole genome oligo microarray chip (4×44K) (Agilent, USA)

2014-07-30 | GSE59897 | GEO

Expression data from Head and neck squamous cell carcinoma cell lines cell line Cal27 after TCF19 knockout mediated by CRISPR/CAS9

Project description:The deletion of TCF19 in CAL27,oral squamous cell carcinoma cell line,was conducted by using CRISPR/Cas9 system. For WT and TCF19 KO CAL27 cell lines, samples were done in triplicates. A total of 3μg of high-quality RNA per sample was used for ribosomal RNA removal by the Epicentre Ribo-zero rRNA Removal Kit (Epicentre, USA) and the sequencing library was prepared using the rRNA-depleted RNA by the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (NEB, USA) following manufacturer’s recommendations. The clustering of the index-coded samples was performed on a cBot Cluster Generation System using TruSeq PE Cluster Kit v3-cBot-HS (Illumina, San Diego, CA, USA), followed by the 150-bp paired-end sequencing on the HiSeq X Ten instrument (Illumina, San Diego, CA, USA) according to the manufacturer’s protocols.

2021-01-01 | GSE153354 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data