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Human NVU - Astrocytes Methamphetamine

ABSTRACT: We used cortical human fetal astrocytes, density 8-15000 cells/cm2 (ScienCell, San Diego, CA, USA seeded separately in Astrocyte medium (ScienCell, San Diego, CA, USA) P0. Methamphetamine (Sigma-Aldrich, St Louis, MO, USA) was prepared at a stock concentration of 10mM in distilled water. Methamphetamine dilutions 50 micromolar were made in Endo and Neuro/Astro/Peri media respectively and applied to the cells for 26 hrs. Further info in "Proteomic and metabolomic characterization of human neurovascular unit cells in response to methamphetamine" Herland et al Adv Biosystems 2020 [doi:10.25345/C55F3P] [dataset license: CC0 1.0 Universal (CC0 1.0)]

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Anna Herland

PROVIDER: MSV000085795 | MassIVE | Tue Jul 21 00:03:00 BST 2020

REPOSITORIES: MassIVE

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