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Physiological and molecular responses to ocean acidification in a model marine symbiosis

ABSTRACT: Pocilloporid corals (Pocillopora acuta) from an upwelling coral reef in Southern Taiwan (Houbihu) were exposed to one of four experimental treatments: ambient temperature (25C)+ambient pCO2 (~430 ppm), ambient temperature+high pCO2 (~850 ppm), high temperature (29C)+ambient pCO2, or high temperature+high pCO2. Proteins were extracted from 2-3 replicates per treatment (n=11 total), digested, and analyzed by iTRAQ followed by nano-liquid chromatography and MS/MS. Since there are only eight iTRAQ labels, 6 and 5 samples were run in each batch ("1" & "2," respectively, in the attached documents), and a normalizer sample was labeled with iTRAQ tag 113 and run in both batches to control for batch-to-batch variation. I am attaching the RAW files, MZML files, MZID files, the P. acuta-Cladocopium transcriptome (conceptually translated to proteins; as a fasta file), a supplemental data (Excel) file, and the associated manuscript, which has not yet been submitted and it subject to change.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Cladocopium Sp. Clade C (ncbitaxon:293275) Pocillopora Acuta (ncbitaxon:1491507)

SUBMITTER: Anderson Mayfield

PROVIDER: MSV000085868 | MassIVE | Sat Aug 01 08:54:00 BST 2020

SECONDARY ACCESSION(S): PXD020681

REPOSITORIES: MassIVE

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