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Site-specific glycosylation mapping of Fc gamma receptor IIIb from neutrophils of individual healthy donors

ABSTRACT: The uploaded dateset corresponds to MS1 and MS2 spectra obtained for Fc gamma receptor IIIb isolated from neutrophils of individual healthy donors. They represent NA1/NA2, NA2/NA2, NA2/NA2 allotype respectively. Based on the data, the targeted analysis of the N-glycosylation has been performed for the article: Wojcik et. al, Site-specific glycosylation mapping of Fc gamma receptor IIIb from neutrophils of individual healthy donors.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Iwona Wojcik

PROVIDER: MSV000085913 | MassIVE | Wed Aug 05 06:07:00 BST 2020

REPOSITORIES: MassIVE

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Site-Specific Glycosylation Mapping of Fc Gamma Receptor IIIb from Neutrophils of Individual Healthy Donors.

Wojcik Iwona I Sénard Thomas T de Graaf Erik L EL Janssen George M C GMC de Ru Arnoud H AH Mohammed Yassene Y van Veelen Peter A PA Vidarsson Gestur G Wuhrer Manfred M Falck David D

Analytical chemistry 20200922 19

Fc gamma receptors (FcγRs) translate antigen recognition by immunoglobulin G (IgG) into various immune responses. A better understanding of this key element of immunity promises novel insights into mechanisms of (auto-/allo-)immune diseases and more rationally designed antibody-based drugs. Glycosylation on both IgG and FcγR impacts their interaction dramatically. Regarding FcγR glycosylation profiling, major analytical challenges are associated with the presence of multiple glycosylation sites ...[more]

PMID: 32886488

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