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A novel mechanism for NF-kB activation via IkB aggregation

ABSTRACT: Proteins which interact with IkBa in insoluble aggregates known as Mallory-Denk bodies were characterized by AP-MS, Biotinylation by antibody recognition (BAR), and LCMS analysis of detergent solubalized aggregates. See the supplementary table (S12) for file annotation.

INSTRUMENT(S): LTQ Orbitrap Velos, Q Exactive Plus

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Maria Almira Correia

PROVIDER: MSV000086042 | MassIVE | Mon Aug 31 12:03:00 BST 2020

REPOSITORIES: MassIVE

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Publications

A Novel Mechanism for NF-κB-activation via IκB-aggregation: Implications for Hepatic Mallory-Denk-Body Induced Inflammation.

Liu Yi Y Trnka Michael J MJ Guan Shenheng S Kwon Doyoung D Kim Do-Hyung DH Chen J-J JJ Greer Peter A PA Burlingame A L AL Correia Maria Almira MA

Molecular & cellular proteomics : MCP 20200910 12

Mallory-Denk-bodies (MDBs) are hepatic protein aggregates associated with inflammation both clinically and in MDB-inducing models. Similar protein aggregation in neurodegenerative diseases also triggers inflammation and NF-κB activation. However, the precise mechanism that links protein aggregation to NF-κB-activation and inflammatory response remains unclear. Herein we find that treating primary hepatocytes with MDB-inducing agents (N-methylprotoporphyrin (NMPP), protoporphyrin IX (PPIX), or Zi ...[more]

PMID: 32912968

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