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Evaluating the performance of 193 nm ultraviolet photodissociaiton (UVPD) for tandem mass tag (TMT) labeled peptides

ABSTRACT: We evaluated 193 nm UVPD for characterization of TMT labeled peptides (HeLa digest) and N-glycopeptides (alpha-1-acid glycoprotein digest) at different conditions, and compared the results with HCD. Samples were digested with trypsin and labeled with TMT6. Instrument data files were searched with either MS-GF+ (using PNNL's DMS processing pipeline) or with Byonic.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Escherichia Coli K-12 (ncbitaxon:83333)

SUBMITTER: Jared B. Shaw

PROVIDER: MSV000086049 | MassIVE | Mon Aug 31 22:55:00 BST 2020

REPOSITORIES: MassIVE

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Evaluating the performance of 193 nm ultraviolet photodissociaiton (UVPD) for tandem mass tag (TMT) labeled peptides

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