Shotgun proteomic analysis of thermally challenged Caribbean reef corals

ABSTRACT: In this work, I took a proteome profiling approach to characterize the cellular biology of 16 coral samples from a laboratory-based thermal stress experiment. The specific goal of this work was to uncover proteins involved in the high-temperature response of the Caribbean reef coral Orbicella faveolata. Coral colonies from two inshore (Cheeca Rocks [UKI] & The Rocks [UKI2]) and one offshore reef (Little Conch [UKO2]) were cored in situ, with cores later brought to the “Experimental Reef Laboratory” housed at the University of Miami’s Rosenstiel School of Marine and Atmospheric Sciences (RSMAS). After laboratory acclimation, the samples were exposed to either 33°C for 5 days or 32°C days for 31 days, with control corals maintained at 30°C. Proteins were extracted from a subset of samples representing multiple coral genotypes exposed to the experimental treatment conditions for 5 or 31 days and sequenced with nano-liquid chromatography followed by mass spectrometry. In this submission, I have uploaded 16 RAW files generated by the mass spectrometer, 16 MZML (open-access mass spectrometry mass peak data) files, 16 MZID (open-access mass spectrometry results) files, and a tab-delineated file that describes which samples were analyzed ("Mayfield et al. online supplemental data file"). I have also uploaded the supplemental material that accompanies the Mayfield et al. (2021) article since that is what readers interested in the specifics of the proteomic analysis pipeline should consult. The peak data were queried against both conceptually translated Orbicella faveolata and Symbiodiniaceae dinoflagellate transcriptomes; both fasta files have been uploaded.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Durusdinium Sp. (ncbitaxon:2486700) Orbicella Faveolata (ncbitaxon:48498) Breviolum Sp. (ncbitaxon:2499526)

SUBMITTER: Anderson Mayfield  

PROVIDER: MSV000086098 | MassIVE |

SECONDARY ACCESSION(S): PXD021349

REPOSITORIES: MassIVE