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Repurposing chloramphenicol acetyltransferase for a robust and efficient designer ester biosynthesis platform

ABSTRACT: Repurposing chloramphenicol acetyltransferase for a robust and efficient designer ester biosynthesis platform

INSTRUMENT(S): Q Exactive Plus

ORGANISM(S): Clostridium Thermocellum Dsm 1313 (ncbitaxon:637887) Hungateiclostridium Thermocellum (ncbitaxon:1515)

SUBMITTER: Richard J. Giannone Cong Trinh

PROVIDER: MSV000086201 | MassIVE |

SECONDARY ACCESSION(S): PXD021695

REPOSITORIES: MassIVE

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Publications

Engineering promiscuity of chloramphenicol acetyltransferase for microbial designer ester biosynthesis.

Seo Hyeongmin H Lee Jong-Won JW Giannone Richard J RJ Dunlap Noah J NJ Trinh Cong T CT

Metabolic engineering 20210416

Robust and efficient enzymes are essential modules for metabolic engineering and synthetic biology strategies across biological systems to engineer whole-cell biocatalysts. By condensing an acyl-CoA and an alcohol, alcohol acyltransferases (AATs) can serve as interchangeable metabolic modules for microbial biosynthesis of a diverse class of ester molecules with broad applications as flavors, fragrances, solvents, and drop-in biofuels. However, the current lack of robust and efficient AATs signif ...[more]

PMID: 33872779

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