Dataset Information

BIN2 and RAPTOR1B mutants phosphoproteomic analysis

ABSTRACT: Finely ground leaf tissue was used. Extraction was done in 8M urea, precipitated with acetone and methanol. Filter-assisted sample preparation (FASP) columns were used to further purify proteins. Purified proteins were reduced with TCEP, alkylated with iodoacetamide and digested into peptides with trypsin and lys-C. Digested peptides were desalted with c18 columns and labelled with 11-plex Tandem Mass Tag (TMT11) reagents. Phosphoenrichment was performed in a two-steps tandem pipeline: High-Select TiO2 Phosphopeptide Enrichment kit (Thermo Scientific) was used first and High Select Fe-NTA Phosphoptide Enrichment kit (Thermo Scientific) was used on the flow-through obtained from first enrichment.

INSTRUMENT(S): Q Exactive Plus

ORGANISM(S): Arabidopsis Thaliana (ncbitaxon:3702)

SUBMITTER: Justin Walley

PROVIDER: MSV000086460 | MassIVE | Tue Nov 17 07:41:00 GMT 2020

REPOSITORIES: MassIVE

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Publications

Integration of multi-omics data reveals interplay between brassinosteroid and Target of Rapamycin Complex signaling in Arabidopsis.

Montes Christian C Wang Ping P Liao Ching-Yi CY Nolan Trevor M TM Song Gaoyuan G Clark Natalie M NM Elmore J Mitch JM Guo Hongqing H Bassham Diane C DC Yin Yanhai Y Walley Justin W JW

The New phytologist 20220811 3

Brassinosteroids (BRs) and Target of Rapamycin Complex (TORC) are two major actors coordinating plant growth and stress responses. Brassinosteroids function through a signaling pathway to extensively regulate gene expression and TORC is known to regulate translation and autophagy. Recent studies have revealed connections between these two pathways, but a system-wide view of their interplay is still missing. We quantified the level of 23 975 transcripts, 11 183 proteins, and 27 887 phosphorylatio ...[more]

PMID: 35892179

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