Proteomics

Dataset Information

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Human testis off-line LC-MS/MS

ABSTRACT: In order to identify more MPs and get deeper proteomic data of testis. We applied high-pH reverse phase (RP) HPLC to fractionate complex samples.On the other hand, to enhance protein coverage, multi-protease strategies were used for 10% SDS-PAGE short separation samples.

INSTRUMENT(S): Q Exactive HF-X

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Ping Xu  

PROVIDER: MSV000086491 | MassIVE | Thu Nov 19 23:03:00 GMT 2020

SECONDARY ACCESSION(S): PXD009737

REPOSITORIES: MassIVE

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Publications

Multiproteases Combined with High-pH Reverse-Phase Separation Strategy Verified Fourteen Missing Proteins in Human Testis Tissue.

Sun Jinshuai J   Shi Jiahui J   Wang Yihao Y   Chen Yang Y   Li Yanchang Y   Kong Degang D   Chang Lei L   Liu Fengsong F   Lv Zhitang Z   Zhou Yue Y   He Fuchu F   Zhang Yao Y   Xu Ping P  

Journal of proteome research 20181016 12

Subsequent to conducting the Chromosome-Centric Human Proteome Project, we have focused on human testis-enriched missing proteins (MPs) since 2015. For protein coverage to be enhanced, a multiprotease strategy was used for separation of samples by 10% SDS-PAGE. For the separating efficiency to be improved, a high-pH reverse phase (RP) separation strategy was applied to fractionate complex samples in this study. A total of 11,558 proteins was identified, which is the largest proteome data set for  ...[more]