Dataset Information

Comparative Proteomic Analysis of English Walnut (Juglans regia L.) Pellicle Tissues Reveal the Regulation of Nut Quality Attributes

ABSTRACT: Walnuts (Juglans regia L.) are a valuable dietary source of polyphenols and lipids, with increasing worldwide consumption. California is a major producer, with Chandler and Tulare among the cultivars more widely grown. Chandler produces kernels with extra light color at a higher frequency than , gaining consumer preference. Here we performed a deep comparative proteome analysis of kernel pellicle tissue from these two valued genotypes, at three harvest maturities, detecting a total of 4,937 J. regia proteins. Late and early maturity stages were compared for each cultivar, revealing many developmental responses common or specific for each cultivar. Top protein biomarkers for each developmental stage were also selected based on larger fold-change differences and lower variance among replicates. Comparison between the genotypes also revealed the common and specific protein repertoires, totaling 321 pellicle proteins with differential abundance. Additionally, in all maturity stages several allergens were among the most abundant proteins, which is of importance for food safety. The proteomics data provides clues on antioxidant, secondary, and hormonal metabolism that could be involved in the loss of quality in the pellicles during processing for commercialization.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Juglans Regia (ncbitaxon:51240)

SUBMITTER: Abhaya M. Dandekar

PROVIDER: MSV000086493 | MassIVE | Fri Nov 20 09:44:00 GMT 2020

SECONDARY ACCESSION(S): PXD022655

REPOSITORIES: MassIVE

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Project description:Molecular events regulating apple fruit ripening and sensory quality are largely unknown. Such knowledge is essential for genomic-assisted apple breeding and postharvest quality management. In this study, a parallel transcriptome profile analysis, scanning electron microscopic (SEM) examination and systematic physiological characterization were performed on two apple cultivars, Honeycrisp (HC) and Cripps Pink (CP), which have distinct ripening features and texture attributes. Systematic physiological characterization of fruit ripening based on weekly maturity data indicated substantial differences in fruit crispness and firmness at comparable ripening stages. SEM images of fruit cortex tissues prepared from fruits with equivalent maturity suggested that the cell wall thickness may contribute to the observed phenotypes of fruit firmness and crispness. A high-density long-oligo apple microarray consisting of duplex 190,135 cross-hybridization-free 50-70-mer isothermal probes, and representing 23,997 UniGene clusters, was manufactured on a Nimblegen array platform. Transcriptome profiling identified a total of 1793 and 1209 UniGene clusters differentially expressed during ripening from cortex tissues of HC and CP, respectively. UniGenes implicated in hormone metabolism and response, cell wall biosynthesis and modification and those encoding transcription factors were among the prominent functional groups. Between the two cultivars, most of the identified UniGenes were similarly regulated during fruit ripening; however, a short list of gene families or specific family members exhibited distinct expression patterns between the two cultivars, which may represent candidate genes regulating cultivar-specific apple fruit ripening patterns and quality attributes. Using a single color labeling system, a total of 24 microarray slides were utilized, one for each cortex tissue sample, for transcriptome profiling analysis. 2 cultivars x 3 developmental stages x 4 biological replicates.

Dataset Information

Comparative Proteomic Analysis of English Walnut (Juglans regia L.) Pellicle Tissues Reveal the Regulation of Nut Quality Attributes

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