Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 1000

Untargeted metabolome profiling of S. cerevisiae predicted peroxisomal genes driven by TEF2 promoter

ABSTRACT: The metabolome profiles of S. cerevisiae mutants where the promoter of genes predicted to code for peroxisomally localized proteins are swapped with the TEF2 promoter were measured by flow-injection analysis.

INSTRUMENT(S): 6550 QTOF (Agilent)

ORGANISM(S): Saccharomyces Cerevisiae (ncbitaxon:4932)

SUBMITTER: Nicola Zamboni

PROVIDER: MSV000086775 | MassIVE | Thu Jan 28 03:10:00 GMT 2021

REPOSITORIES: MassIVE

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

GNPS Untargeted metabolome profiling of S. cerevisiae predicted peroxisomal genes driven by TEF2 promoter

Project description:The metabolome profiles of S. cerevisiae mutants where the promoter of genes predicted to code for peroxisomally localized proteins are swapped with the TEF2 promoter were measured by flow-injection analysis.

2021-01-28 | MSV000086775 | GNPS

Untargeted metabolome profiling of S. cerevisiae loss-of-function mutants in predicted peroxisomal genes

Project description:The metabolome profiles of S. cerevisiae loss-of-function mutants in genes predicted to code for peroxisomally localized proteins were measured by flow-injection analysis.

2021-01-27 | MSV000086773 | MassIVE

GNPS Untargeted metabolome profiling of S. cerevisiae loss-of-function mutants in predicted peroxisomal genes

Project description:The metabolome profiles of S. cerevisiae loss-of-function mutants in genes predicted to code for peroxisomally localized proteins were measured by flow-injection analysis.

2021-01-27 | MSV000086773 | GNPS

Lipidomic analysis of mutants in predicted peroxisomal Saccharomyces cerevisiae genes

Project description:Lipid extracts from a range of mutants in genes whose gene-products are predicted to localize to the peroxisome were profiled using a 2 minute gradient liquid chromatography method and annotation of lipids based on MS1.

2021-01-28 | MSV000086777 | MassIVE

Single-nucleosome mapping of histone modifications in S. cerevisiae

Project description:We have used a high-resolution tiled microarray, with single-nucleosome resolution, to investigate the in vivo occurrence of combinations of 12 histone modifications on thousands of nucleosomes in actively growing S. cerevisiae. We found that histone modifications do not occur independently; there are roughly two groups of co-occurring modifications. We find no evidence for a deterministic code of many discrete states, but instead see blended, continuous patterns that distinguish nucleosomes at one location (promoter nucleosomes, for example) from those at another location (over the 3’ ends of coding regions, for example). These results are consistent with the idea of a simple, redundant histone code, in which multiple modifications share the same role. Keywords: ChIP-chip

2005-09-01 | GSE2954 | GEO

Quantification of proline to serine mistranslation in a Saccharomyces cerevisiae strain expressing an inducible tRNA variant

Project description:Altering the genetic code for applications in synthetic biology and genetic code expansion involves engineered tRNAs that incorporate amino acids that differ from what is defined by the “standard” genetic code. Since these engineered tRNA variants can be lethal due to proteotoxic stress, regulating their expression is necessary to achieve high levels of the resulting novel proteins. Mechanisms to positively regulate transcription with exogenous activator proteins like those often used to regulate RNA polymerase II (RNAP II) transcribed genes are not applicable to tRNAs as their expression by RNA polymerase III requires elements internal to the tRNA. Here, we show that tRNA expression is repressed by overlapping transcription from an adjacent RNAP II promoter. Regulating the expression of the RNAP II promoter allows inverse regulation of the tRNA. Placing either Gal4 or TetR-VP16 activated promoters downstream of a mistranslating tRNA serine variant that mis-incorporates serine at proline codons in Saccharomyces cerevisiae allows mistranslation at a level not otherwise possible because of the toxicity of the unregulated tRNA. Using mass spectrometry, we determine th frequency of mistranslation in both the induced and repressed conditions of the galactose inducible and tetracycline inducible systems.

2021-11-03 | PXD028496 | Pride

GNPS Lipidomic analysis of mutants in predicted peroxisomal Saccharomyces cerevisiae genes

2021-01-28 | MSV000086777 | GNPS

Saccharomyces cerevisiae

Project description:Saccharomyces cerevisiae promoter library

| PRJNA200900 | ENA

Transcription profiling of S. cerevisiae ribosomal protein knockouts

Project description:Duplicated genes escape gene loss by conferring a dosage benefit or evolving diverged functions. The yeast Saccharomyces cerevisiae contains many duplicated genes encoding ribosomal proteins. Prior studies have suggested that these duplicated proteins are functionally redundant and affect cellular processes in proportion to their expression. In contrast, through studies of ASH1 mRNA in yeast, we demonstrate paralog-specific requirements for the translation of localized mRNAs. Intriguingly, these paralog-specific effects are limited to a distinct subset of duplicated ribosomal proteins. Moreover, transcriptional and phenotypic profiling of cells lacking specific ribosomal proteins reveals differences between the functional roles of ribosomal protein paralogs that extend beyond effects on mRNA localization. Finally, we show that ribosomal protein paralogs exhibit differential requirements for assembly and localization. Together, our data indicate complex specialization of ribosomal proteins for specific cellular processes, and support the existence of a ribosomal code. Experiment Overall Design: We used Affymetrix arrays to analyze the transcriptional profiles of cells lacking certain duplicated ribosomal protein genes in order to determine if paralogous ribosomal proteins have differing cellular effects and roles. Experiment Overall Design: Two biological replicates were performed for each strain. Each ribosomal protein deletion was then compared to the isogenic wild-type strain.

2008-06-16 | E-GEOD-8761 | biostudies-arrayexpress

Metabolome profiling of induced overexpression of S cerevisiae genes

Project description:Metabolite extracts were collected for 86 inducible overexpression strains in Saccharomyces cerevisiae in the presence and absence of inducer. Induction was carried out for 1.5 hours prior to metabolite extraction. Extracts were subjected to flow injection analysis.

2021-09-20 | MSV000088125 | MassIVE

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data