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Aureobasidium pullulans biocontrol mechanisms; genome, transcriptome & secretome analyses

ABSTRACT: Aureobasidium pullulans is being studied with respect to its biotechnological applications in the degradation and modification of lignocellulose substrates or the production of the polysaccharide pullulan. In addition, the species is also used as a commercial plant protection agent against the bacterial pome fruit disease fireblight or against fungal postharvest diseases of fruits. The A. pullulans strain NBB 7.2.1 was originally isolated from a soil sample (from a Swiss orchard), but it was shown to be more competitive on apples than in soil (Gross et al., 2018). The antifungal activity of the isolate NBB 7.2.1 against fungal plant pathogens, and filamentous fungi in general, was assessed: Among 40 different yeasts, it belonged to the most strongly antifungal isolates (Hilber-Bodmer et al., 2017). The genome of A. pullulans NBB 7.2.1 was sequenced and de novo assembled by Agroscope using a combination of long reads from Pacific Biosciences Sequel technology and Illumina MiSeq short read data and subsequently annotated by JGI. The high quality genome sequence (comprising 12 chromosomes and one circular mitogenome) serves as the foundation for identifying the underlying molecular mechanisms that confer antifungal activity and to determine which factors may be targeted to improve the reliability and efficacy of A. pullulans as a plant protection agent in the field and under storage conditions. This dataset contains raw reads for four replicates of three fungal culture supernatants B1 Files: A_pul_F_ox: Interaction of A. pullulans and F. oxysporum in peptone buffer B2 Files: Aureobasidium: Pure culture of A. pullulans in peptone buffer B3 Files: Fusarium: Pure culture of F. oxysporum in peptone buffer

INSTRUMENT(S): Q-Exactive HFX

ORGANISM(S): Fusarium Oxysporum F. Sp. Radicis-lycopersici (ncbitaxon:61372) Aureobasidium Pullulans Var. Pullulans (ncbitaxon:178873)

SUBMITTER: Florian Freimoser

PROVIDER: MSV000086991 | MassIVE | Wed Mar 03 11:34:00 GMT 2021

SECONDARY ACCESSION(S): PXD024503

REPOSITORIES: MassIVE

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Project description:Pneumonia is a serious problem worldwide. We recently demonstrated that innate defense mechanisms of the lung are highly inducible against pneumococcal pneumonia. To determine the breadth of protection conferred by stimulation of lung mucosal innate immunity, and to identify cells and signaling pathways activated by this treatment, mice were treated with an aerosolized bacterial lysate, then challenged with lethal doses of bacterial and fungal pathogens. Mice were highly protected against a broad array of Gram-positive, Gram-negative, and Class A bioterror bacterial pathogens, and Aspergillus fumigatus. Protection was associated with rapid pathogen killing within the lungs, and this effect was recapitulated in vitro using a respiratory epithelial cell line. Gene expression analysis of lung tissue showed marked activation of NF-kappa-B, Type I and II interferon, and antifungal Card9-Bcl10-Malt1 pathways. Cytokines were the most strongly induced genes, but the inflammatory cytokines TNF and IL-6 were not required for protection. Lung-expressed antimicrobial peptides were also highly upregulated. Taken together, stimulated innate resistance (StIR) appears to occur through the activation of multiple host defense signaling pathways in lung epithelial cells, inducing rapid pathogen killing, and conferring broad protection against virulent bacterial and fungal pathogens. Augmentation of innate antimicrobial defenses of the lungs might have therapeutic value for protection of patients with neutropenia or impaired adaptive immunity against opportunistic pneumonia, and for defense of immunocompetent subjects against a bioterror threat or epidemic respiratory infection. Keywords: Differential expression, innate immunity, pneumonia, immunocompromised host; lung epithelium, in vitro, MLE-15 cells were treated with sham (PBS), NTHi lysate (100 ug/ml) or EF2505-III (40 ug/ml). 4 unique samples per group. Treated for 2 hours. Hybridized to Illumina Sentrix Mouse-6 v1.1 Beadhips.

Project description:Pneumonia is a serious problem worldwide. We recently demonstrated that innate defense mechanisms of the lung are highly inducible against pneumococcal pneumonia. To determine the breadth of protection conferred by stimulation of lung mucosal innate immunity, and to identify cells and signaling pathways activated by this treatment, mice were treated with an aerosolized bacterial lysate, then challenged with lethal doses of bacterial and fungal pathogens. Mice were highly protected against a broad array of Gram-positive, Gram-negative, and Class A bioterror bacterial pathogens, and Aspergillus fumigatus. Protection was associated with rapid pathogen killing within the lungs, and this effect was recapitulated in vitro using a respiratory epithelial cell line. Gene expression analysis of lung tissue showed marked activation of NF-kappaB, Type I and II interferon, and antifungal Card9-Bcl10-Malt1 pathways. Cytokines were the most strongly induced genes, but the inflammatory cytokines TNF and IL-6 were not required for protection. Lung-expressed antimicrobial peptides were also highly upregulated. Taken together, stimulated innate resistance (StIR) appears to occur through the activation of multiple host defense signaling pathways in lung epithelial cells, inducing rapid pathogen killing, and conferring broad protection against virulent bacterial and fungal pathogens. Augmentation of innate antimicrobial defenses of the lungs might have therapeutic value for protection of patients with neutropenia or impaired adaptive immunity against opportunistic pneumonia, and for defense of immunocompetent subjects against a bioterror threat or epidemic respiratory infection. Keywords: differential gene expression; time course; innate immunity; pneumonia; immunocompromised host; lung epithelium Gene expression patterns in mouse lung homogenates were analyzed 2h after exposure to aerosolized PBS (Sham treatment), 2h after exposure to aerosolized NTHi lysate or 4h after exposure to aerosolized NTHi lysate. Each group consisted of six mice.

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Aureobasidium pullulans biocontrol mechanisms; genome, transcriptome & secretome analyses

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