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The Histone H1-Like Protein AlgP Facilitates Even Spacing of Polyphosphate Granules in Pseudomonas aeruginosa

ABSTRACT: Nitrogen starvation results in polyphosphate accumulation in Pseudomonas aeruginosa. Using Percoll gradient centrifugation, we pelleted polyphosphate granules and compared their composition to the unfractionated cell lysate using mass spectrometry. Among the enriched proteins was the alginate regulatory protein AlgP. Its unusual amino-acid composition, overall charge, and physicochemical properties lead us to further investigate its role in granule association and spacing in Pseudomonas aeruginosa.

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Pseudomonas Aeruginosa Ucbpp-pa14 (ncbitaxon:208963)

SUBMITTER: Lisa Racki James R. Williamson

PROVIDER: MSV000087218 | MassIVE | Fri Apr 16 12:38:00 BST 2021

SECONDARY ACCESSION(S): PXD025444

REPOSITORIES: MassIVE

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