Dataset Information

Proteomic analysis of Fusarium sp. DS 682 Fungal Mineral Weathering in a Mineral Doped Soil Micromodel

ABSTRACT: Cell lysates of Fusarium sp. DS 682, a saprotrophic fungus, were prepared from fungal biomass grown on PDA agar without M9 and micronutrients. Fungus was exposed to minerals, 100 uL of 50% (w/v) natural kaolinite solution. Three fungal plate collections for each growth condition, with (+ mineral) and without mineral (- mineral), were grown for 14 days on PDA (incubated at 28 C) and harvested after 15 days. Extracted hyphae (500 mg) was prepared using the MPLEx protocol. A nanoACQUITY ultra performance liquid chromatography (LC) with a 2DLC system was used for separation of protein digests. Eluted peptides from the C18 column were analyzed using a Q-Exactive Plus Orbitrap MS for high resolution MS and high-energy collision-induced dissociation tandem MS by electrospray ionization for subsequent quantitative proteomic analysis. Data was searched with MaxQuant. Additional post-processed results and metadata can be accessed at https://doi.org/10.25584/KSOmicsFspDS682/1766303

INSTRUMENT(S): Q Exactive Plus

ORGANISM(S): Fusarium Sp. (ncbitaxon:29916)

SUBMITTER: Christopher R. Anderton

PROVIDER: MSV000087221 | MassIVE | Fri Apr 16 16:05:00 BST 2021

REPOSITORIES: MassIVE

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Publications

A Mineral-Doped Micromodel Platform Demonstrates Fungal Bridging of Carbon Hot Spots and Hyphal Transport of Mineral-Derived Nutrients.

Bhattacharjee Arunima A Qafoku Odeta O Richardson Jocelyn A JA Anderson Lindsey N LN Schwarz Kaitlyn K Bramer Lisa M LM Lomas Gerard X GX Orton Daniel J DJ Zhu Zihua Z Engelhard Mark H MH Bowden Mark E ME Nelson William C WC Jumpponen Ari A Jansson Janet K JK Hofmockel Kirsten S KS Anderton Christopher R CR

mSystems 20221117 6

Soil fungi facilitate the translocation of inorganic nutrients from soil minerals to other microorganisms and plants. This ability is particularly advantageous in impoverished soils because fungal mycelial networks can bridge otherwise spatially disconnected and inaccessible nutrient hot spots. However, the molecular mechanisms underlying fungal mineral weathering and transport through soil remains poorly understood primarily due to the lack of a platform for spatially resolved analysis of bioti ...[more]

PMID: 36394319

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